Impaired Phagolysosomal Fusion of Peripheral Blood Monocytes from HIV‐Infected Subjects
We evaluated phagolysosomal fusion in peripheral blood monocytes from 20 HIV‐infected individuals and 40 normal controls, using a fluorescence assay with acridine orange as marker. The percentages of phagolysosomal fusion of monocytes from HIV‐infected subjects, after 30 and 60 min of yeast ingestio...
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Veröffentlicht in: | Scandinavian journal of immunology 1993-11, Vol.38 (5), p.423-427 |
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description | We evaluated phagolysosomal fusion in peripheral blood monocytes from 20 HIV‐infected individuals and 40 normal controls, using a fluorescence assay with acridine orange as marker. The percentages of phagolysosomal fusion of monocytes from HIV‐infected subjects, after 30 and 60 min of yeast ingestion, (mean ± standard deviation) 57·2 ± 17 and 63·2 ± 18·6, respectively, when compared to normal controls (72·4 ± 7·8 and 77 ± 8·1), did not differ significantly. However, there was a direct linear association between the percentages of phagolysosomal fusion and CD4+ lymphocytes (P |
doi_str_mv | 10.1111/j.1365-3083.1993.tb02583.x |
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G. ; STERNIK, G. ; SEN, L. ; DIEZ, R. A. ; PLANES, N. ; PIROLA, D. ; ESTEVEZ, M. E.</creator><creatorcontrib>PITTIS, M. G. ; STERNIK, G. ; SEN, L. ; DIEZ, R. A. ; PLANES, N. ; PIROLA, D. ; ESTEVEZ, M. E.</creatorcontrib><description>We evaluated phagolysosomal fusion in peripheral blood monocytes from 20 HIV‐infected individuals and 40 normal controls, using a fluorescence assay with acridine orange as marker. The percentages of phagolysosomal fusion of monocytes from HIV‐infected subjects, after 30 and 60 min of yeast ingestion, (mean ± standard deviation) 57·2 ± 17 and 63·2 ± 18·6, respectively, when compared to normal controls (72·4 ± 7·8 and 77 ± 8·1), did not differ significantly. However, there was a direct linear association between the percentages of phagolysosomal fusion and CD4+ lymphocytes (P<0·001) or CD4/CD8 T‐cell ratio (P<0·01). These results suggest that phagolysosomal dysfunction becomes evident at late stages of HIV infection and progresses as CD4+ T‐lymphocyte count and CD4/CD8 T‐cell ratio decrease. On the other hand, recombinant gp120 inhibited significantly normal phagolysosomal fusion at concentrations ranging between 1 and 1000 ng/ml. Taking together the results obtained, we can conclude that gp120 could be responsible for monocyte phagolysosomal dysfunction observed in HIV infected patients.</description><identifier>ISSN: 0300-9475</identifier><identifier>EISSN: 1365-3083</identifier><identifier>DOI: 10.1111/j.1365-3083.1993.tb02583.x</identifier><identifier>PMID: 8235445</identifier><identifier>CODEN: SJIMAX</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; AIDS/HIV ; Biological and medical sciences ; CD4-CD8 Ratio ; Cell Fusion ; Female ; HIV Envelope Protein gp120 - pharmacology ; HIV Infections - blood ; HIV Infections - pathology ; HIV-1 ; human immunodeficiency virus ; Humans ; Immunodeficiencies ; Immunodeficiencies. Immunoglobulinopathies ; Immunopathology ; Lysosomes - pathology ; Lysosomes - physiology ; Male ; Medical sciences ; Middle Aged ; Monocytes - physiology ; Phagocytosis ; Phagosomes - pathology ; Phagosomes - physiology ; Recombinant Proteins - pharmacology</subject><ispartof>Scandinavian journal of immunology, 1993-11, Vol.38 (5), p.423-427</ispartof><rights>1994 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4293-6296b9f546c2166a0107ea3b7e45af7863dd6ddc710eace98db9828d8c72f1603</citedby><cites>FETCH-LOGICAL-c4293-6296b9f546c2166a0107ea3b7e45af7863dd6ddc710eace98db9828d8c72f1603</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-3083.1993.tb02583.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-3083.1993.tb02583.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3779066$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8235445$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>PITTIS, M. G.</creatorcontrib><creatorcontrib>STERNIK, G.</creatorcontrib><creatorcontrib>SEN, L.</creatorcontrib><creatorcontrib>DIEZ, R. A.</creatorcontrib><creatorcontrib>PLANES, N.</creatorcontrib><creatorcontrib>PIROLA, D.</creatorcontrib><creatorcontrib>ESTEVEZ, M. E.</creatorcontrib><title>Impaired Phagolysosomal Fusion of Peripheral Blood Monocytes from HIV‐Infected Subjects</title><title>Scandinavian journal of immunology</title><addtitle>Scand J Immunol</addtitle><description>We evaluated phagolysosomal fusion in peripheral blood monocytes from 20 HIV‐infected individuals and 40 normal controls, using a fluorescence assay with acridine orange as marker. The percentages of phagolysosomal fusion of monocytes from HIV‐infected subjects, after 30 and 60 min of yeast ingestion, (mean ± standard deviation) 57·2 ± 17 and 63·2 ± 18·6, respectively, when compared to normal controls (72·4 ± 7·8 and 77 ± 8·1), did not differ significantly. However, there was a direct linear association between the percentages of phagolysosomal fusion and CD4+ lymphocytes (P<0·001) or CD4/CD8 T‐cell ratio (P<0·01). These results suggest that phagolysosomal dysfunction becomes evident at late stages of HIV infection and progresses as CD4+ T‐lymphocyte count and CD4/CD8 T‐cell ratio decrease. On the other hand, recombinant gp120 inhibited significantly normal phagolysosomal fusion at concentrations ranging between 1 and 1000 ng/ml. Taking together the results obtained, we can conclude that gp120 could be responsible for monocyte phagolysosomal dysfunction observed in HIV infected patients.</description><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>CD4-CD8 Ratio</subject><subject>Cell Fusion</subject><subject>Female</subject><subject>HIV Envelope Protein gp120 - pharmacology</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - pathology</subject><subject>HIV-1</subject><subject>human immunodeficiency virus</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Lysosomes - pathology</subject><subject>Lysosomes - physiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Monocytes - physiology</subject><subject>Phagocytosis</subject><subject>Phagosomes - pathology</subject><subject>Phagosomes - physiology</subject><subject>Recombinant Proteins - pharmacology</subject><issn>0300-9475</issn><issn>1365-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUctKxDAUDaLo-PgEoYi465hHmzQuBBUfFUXBB7gKaR7aoW3GZIrOzk_wG_0SW6bMVryb3Jtz7kk4B4A9BMeoq8PJGBGaxgRmZIw4J-NZAXHaDZ8rYLSEVsEIEghjnrB0A2yGMIEQEczIOljPMEmTJB2Bl7yeytIbHd2_yVdXzYMLrpZVdNGG0jWRs9G98eX0zfju8rRyTke3rnFqPjMhst7V0VX-_PP1nTfWqFmn89AWk64L22DNyiqYneHcAk8X549nV_HN3WV-dnITqwRzElPMacFtmlCFEaUSIsiMJAUzSSotyyjRmmqtGIJGKsMzXfAMZzpTDFtEIdkCBwvdqXfvrQkzUZdBmaqSjXFtEIzCFGLE_iQiyimjrFc8WhCVdyF4Y8XUl7X0c4Gg6AMQE9G7LHqXRR-AGAIQn93y7vBKW9RGL1cHxzt8f8BlULKyXjaqDEsaYYxDSjva8YL2UVZm_o8PiIfrPMGE_AKboaNY</recordid><startdate>199311</startdate><enddate>199311</enddate><creator>PITTIS, M. G.</creator><creator>STERNIK, G.</creator><creator>SEN, L.</creator><creator>DIEZ, R. A.</creator><creator>PLANES, N.</creator><creator>PIROLA, D.</creator><creator>ESTEVEZ, M. E.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>199311</creationdate><title>Impaired Phagolysosomal Fusion of Peripheral Blood Monocytes from HIV‐Infected Subjects</title><author>PITTIS, M. G. ; STERNIK, G. ; SEN, L. ; DIEZ, R. A. ; PLANES, N. ; PIROLA, D. ; ESTEVEZ, M. E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4293-6296b9f546c2166a0107ea3b7e45af7863dd6ddc710eace98db9828d8c72f1603</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adult</topic><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>CD4-CD8 Ratio</topic><topic>Cell Fusion</topic><topic>Female</topic><topic>HIV Envelope Protein gp120 - pharmacology</topic><topic>HIV Infections - blood</topic><topic>HIV Infections - pathology</topic><topic>HIV-1</topic><topic>human immunodeficiency virus</topic><topic>Humans</topic><topic>Immunodeficiencies</topic><topic>Immunodeficiencies. Immunoglobulinopathies</topic><topic>Immunopathology</topic><topic>Lysosomes - pathology</topic><topic>Lysosomes - physiology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Monocytes - physiology</topic><topic>Phagocytosis</topic><topic>Phagosomes - pathology</topic><topic>Phagosomes - physiology</topic><topic>Recombinant Proteins - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>PITTIS, M. G.</creatorcontrib><creatorcontrib>STERNIK, G.</creatorcontrib><creatorcontrib>SEN, L.</creatorcontrib><creatorcontrib>DIEZ, R. A.</creatorcontrib><creatorcontrib>PLANES, N.</creatorcontrib><creatorcontrib>PIROLA, D.</creatorcontrib><creatorcontrib>ESTEVEZ, M. 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E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Impaired Phagolysosomal Fusion of Peripheral Blood Monocytes from HIV‐Infected Subjects</atitle><jtitle>Scandinavian journal of immunology</jtitle><addtitle>Scand J Immunol</addtitle><date>1993-11</date><risdate>1993</risdate><volume>38</volume><issue>5</issue><spage>423</spage><epage>427</epage><pages>423-427</pages><issn>0300-9475</issn><eissn>1365-3083</eissn><coden>SJIMAX</coden><abstract>We evaluated phagolysosomal fusion in peripheral blood monocytes from 20 HIV‐infected individuals and 40 normal controls, using a fluorescence assay with acridine orange as marker. The percentages of phagolysosomal fusion of monocytes from HIV‐infected subjects, after 30 and 60 min of yeast ingestion, (mean ± standard deviation) 57·2 ± 17 and 63·2 ± 18·6, respectively, when compared to normal controls (72·4 ± 7·8 and 77 ± 8·1), did not differ significantly. However, there was a direct linear association between the percentages of phagolysosomal fusion and CD4+ lymphocytes (P<0·001) or CD4/CD8 T‐cell ratio (P<0·01). These results suggest that phagolysosomal dysfunction becomes evident at late stages of HIV infection and progresses as CD4+ T‐lymphocyte count and CD4/CD8 T‐cell ratio decrease. On the other hand, recombinant gp120 inhibited significantly normal phagolysosomal fusion at concentrations ranging between 1 and 1000 ng/ml. Taking together the results obtained, we can conclude that gp120 could be responsible for monocyte phagolysosomal dysfunction observed in HIV infected patients.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8235445</pmid><doi>10.1111/j.1365-3083.1993.tb02583.x</doi><tpages>5</tpages></addata></record> |
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subjects | Adult AIDS/HIV Biological and medical sciences CD4-CD8 Ratio Cell Fusion Female HIV Envelope Protein gp120 - pharmacology HIV Infections - blood HIV Infections - pathology HIV-1 human immunodeficiency virus Humans Immunodeficiencies Immunodeficiencies. Immunoglobulinopathies Immunopathology Lysosomes - pathology Lysosomes - physiology Male Medical sciences Middle Aged Monocytes - physiology Phagocytosis Phagosomes - pathology Phagosomes - physiology Recombinant Proteins - pharmacology |
title | Impaired Phagolysosomal Fusion of Peripheral Blood Monocytes from HIV‐Infected Subjects |
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