Impaired Phagolysosomal Fusion of Peripheral Blood Monocytes from HIV‐Infected Subjects

We evaluated phagolysosomal fusion in peripheral blood monocytes from 20 HIV‐infected individuals and 40 normal controls, using a fluorescence assay with acridine orange as marker. The percentages of phagolysosomal fusion of monocytes from HIV‐infected subjects, after 30 and 60 min of yeast ingestio...

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Veröffentlicht in:Scandinavian journal of immunology 1993-11, Vol.38 (5), p.423-427
Hauptverfasser: PITTIS, M. G., STERNIK, G., SEN, L., DIEZ, R. A., PLANES, N., PIROLA, D., ESTEVEZ, M. E.
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container_issue 5
container_start_page 423
container_title Scandinavian journal of immunology
container_volume 38
creator PITTIS, M. G.
STERNIK, G.
SEN, L.
DIEZ, R. A.
PLANES, N.
PIROLA, D.
ESTEVEZ, M. E.
description We evaluated phagolysosomal fusion in peripheral blood monocytes from 20 HIV‐infected individuals and 40 normal controls, using a fluorescence assay with acridine orange as marker. The percentages of phagolysosomal fusion of monocytes from HIV‐infected subjects, after 30 and 60 min of yeast ingestion, (mean ± standard deviation) 57·2 ± 17 and 63·2 ± 18·6, respectively, when compared to normal controls (72·4 ± 7·8 and 77 ± 8·1), did not differ significantly. However, there was a direct linear association between the percentages of phagolysosomal fusion and CD4+ lymphocytes (P
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G. ; STERNIK, G. ; SEN, L. ; DIEZ, R. A. ; PLANES, N. ; PIROLA, D. ; ESTEVEZ, M. E.</creator><creatorcontrib>PITTIS, M. G. ; STERNIK, G. ; SEN, L. ; DIEZ, R. A. ; PLANES, N. ; PIROLA, D. ; ESTEVEZ, M. E.</creatorcontrib><description>We evaluated phagolysosomal fusion in peripheral blood monocytes from 20 HIV‐infected individuals and 40 normal controls, using a fluorescence assay with acridine orange as marker. The percentages of phagolysosomal fusion of monocytes from HIV‐infected subjects, after 30 and 60 min of yeast ingestion, (mean ± standard deviation) 57·2 ± 17 and 63·2 ± 18·6, respectively, when compared to normal controls (72·4 ± 7·8 and 77 ± 8·1), did not differ significantly. However, there was a direct linear association between the percentages of phagolysosomal fusion and CD4+ lymphocytes (P&lt;0·001) or CD4/CD8 T‐cell ratio (P&lt;0·01). These results suggest that phagolysosomal dysfunction becomes evident at late stages of HIV infection and progresses as CD4+ T‐lymphocyte count and CD4/CD8 T‐cell ratio decrease. On the other hand, recombinant gp120 inhibited significantly normal phagolysosomal fusion at concentrations ranging between 1 and 1000 ng/ml. 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Taking together the results obtained, we can conclude that gp120 could be responsible for monocyte phagolysosomal dysfunction observed in HIV infected patients.</description><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>CD4-CD8 Ratio</subject><subject>Cell Fusion</subject><subject>Female</subject><subject>HIV Envelope Protein gp120 - pharmacology</subject><subject>HIV Infections - blood</subject><subject>HIV Infections - pathology</subject><subject>HIV-1</subject><subject>human immunodeficiency virus</subject><subject>Humans</subject><subject>Immunodeficiencies</subject><subject>Immunodeficiencies. Immunoglobulinopathies</subject><subject>Immunopathology</subject><subject>Lysosomes - pathology</subject><subject>Lysosomes - physiology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Monocytes - physiology</subject><subject>Phagocytosis</subject><subject>Phagosomes - pathology</subject><subject>Phagosomes - physiology</subject><subject>Recombinant Proteins - pharmacology</subject><issn>0300-9475</issn><issn>1365-3083</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVUctKxDAUDaLo-PgEoYi465hHmzQuBBUfFUXBB7gKaR7aoW3GZIrOzk_wG_0SW6bMVryb3Jtz7kk4B4A9BMeoq8PJGBGaxgRmZIw4J-NZAXHaDZ8rYLSEVsEIEghjnrB0A2yGMIEQEczIOljPMEmTJB2Bl7yeytIbHd2_yVdXzYMLrpZVdNGG0jWRs9G98eX0zfju8rRyTke3rnFqPjMhst7V0VX-_PP1nTfWqFmn89AWk64L22DNyiqYneHcAk8X549nV_HN3WV-dnITqwRzElPMacFtmlCFEaUSIsiMJAUzSSotyyjRmmqtGIJGKsMzXfAMZzpTDFtEIdkCBwvdqXfvrQkzUZdBmaqSjXFtEIzCFGLE_iQiyimjrFc8WhCVdyF4Y8XUl7X0c4Gg6AMQE9G7LHqXRR-AGAIQn93y7vBKW9RGL1cHxzt8f8BlULKyXjaqDEsaYYxDSjva8YL2UVZm_o8PiIfrPMGE_AKboaNY</recordid><startdate>199311</startdate><enddate>199311</enddate><creator>PITTIS, M. 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identifier ISSN: 0300-9475
ispartof Scandinavian journal of immunology, 1993-11, Vol.38 (5), p.423-427
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source MEDLINE; Wiley Online Library Journals Frontfile Complete
subjects Adult
AIDS/HIV
Biological and medical sciences
CD4-CD8 Ratio
Cell Fusion
Female
HIV Envelope Protein gp120 - pharmacology
HIV Infections - blood
HIV Infections - pathology
HIV-1
human immunodeficiency virus
Humans
Immunodeficiencies
Immunodeficiencies. Immunoglobulinopathies
Immunopathology
Lysosomes - pathology
Lysosomes - physiology
Male
Medical sciences
Middle Aged
Monocytes - physiology
Phagocytosis
Phagosomes - pathology
Phagosomes - physiology
Recombinant Proteins - pharmacology
title Impaired Phagolysosomal Fusion of Peripheral Blood Monocytes from HIV‐Infected Subjects
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