Self-association of sodium cholate in isotonic saline solutions

The self-association of dialyzed solutions of sodium cholate in isotonic saline solutions has been studied by vapor pressure osmometry and sedimentation equilibrium. These studies were carried out at 25, 31 and 37°C. In all experiments the self-association could be described as a two-equilibrium constant, indefinite self-association in which odd species beyond monomer were absent. The plots of M 1/ M na or M 1/ M wa vs. c were quite smooth with no sharp breaks; this suggested that there were no critical phenomena. The temperature dependence of the self-association was quite small. Our results are in accord with other studies on sodium cholate which indicate that the self-association involves several species, and that it is not a monomer- n-mer self-association.