Superior resolution of γ-crystallins from microdissected eye lens by cation-exchange high-performance liquid chromatography

A novel procedure is presented for the rapid quantitative analysis of eye lens γ-crystallins and β s-crystallin by ion-exchange high-performance liquid chromatography on Synchropak CM300. At least six different γ-crystallin gene products can be resolved from the soluble fraction of calf lens extract...

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Veröffentlicht in:	Biochemical and biophysical research communications 1985-02, Vol.127 (1), p.153-160
Hauptverfasser:	Siezen, Roland J., Kaplan, Elizabeth D., Anello, Robert D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Applied sciences Cattle Chromatography, High Pressure Liquid - methods crystallin Crystallins - analysis Exact sciences and technology eye lens Hydrogen-Ion Concentration ion-exchange chromatography Lens, Crystalline - analysis Other techniques and industries Time Factors
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Beschreibung
Zusammenfassung:	A novel procedure is presented for the rapid quantitative analysis of eye lens γ-crystallins and β s-crystallin by ion-exchange high-performance liquid chromatography on Synchropak CM300. At least six different γ-crystallin gene products can be resolved from the soluble fraction of calf lens extract. This method is applicable to the analysis of microsections from individual lenses, and can be used to rapidly characterize spatial variations in γ-crystallin composition which occur with aging and cataractogenesis.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/S0006-291X(85)80138-8