Polymerase chain reaction with the 30-kb circular plasmid of Borrelia burgdorferi B31 as a target for detection of the Lyme borreliosis agents in cerebrospinal fluid
The polymerase chain reaction (PCR) was developed for use in the detection of Borrelia burgdorferi sensu lato, the Lyme disease agent. A 333-bp fragment of the 30-kbp circular plasmid from Borrelia burgdorferi B31 was amplified and PCR products were analysed by DNA-DNA hybridization. Sensitivity was...
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| Veröffentlicht in: | Research in microbiology 1993-03, Vol.144 (3), p.211-219 |
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| creator | Amouriaux, P. Assous, M. Margarita, D. Baranton, G. Girons, I.Saint |
| description | The polymerase chain reaction (PCR) was developed for use in the detection of
Borrelia burgdorferi sensu lato, the Lyme disease agent. A 333-bp fragment of the 30-kbp circular plasmid from
Borrelia burgdorferi B31 was amplified and PCR products were analysed by DNA-DNA hybridization. Sensitivity was enhanced by addition of a carrier to the samples before treatment and enabled detection of as few as 1 to 10 bacteria. Specific products were obtained only with the lyme disease agents, but not with other spirochetes or unrelated bacteria.
B. burgdorferi sensu lato was detected in cerebrospinal fluid (CSF) from 11 out of 45 patients with confirmed Lyme neuroborreliosis. In a prospective study, 20 out of 315 CSF samples from potential patients were PCR-positive. Forty uninfected patients were PCR-negative.
Un test d'amplification génique (PCR) a été développé pour la détection de
Borrelia burgdorferi sensu lato, agents de la maladie de Lyme. Un fragment de 333 bp provenant du palsmide circulaire de 30 kb de
B. burgdorferi B31 a été amplifié et les produits d'amplification analysis analysis par hybridation ADN/ADN. L'addition d'un entraîneur aux échantillons avant traitement a permis d'augmenter la sen sibilité Des produits spécifiques ont été obtenus uniquement avec les agents de la maladie de Lyme et pas avec d'autres spirochètes ou d'autres bactéries plus éloighées.
B. burgdorferi sensu lato a été détectée dans le liquide céphalorachidien (LCR) de ll patients sur 45 dont la neuroborréliose de Lyme avait été confirmée. Dans une étude prospective, 20 des 315 énhantillons de LCR étaient PCR
+; 40 patients non infectés étaient PCR
−. |
| doi_str_mv | 10.1016/0923-2508(93)90046-5 |
| format | Article |
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Borrelia burgdorferi sensu lato, the Lyme disease agent. A 333-bp fragment of the 30-kbp circular plasmid from
Borrelia burgdorferi B31 was amplified and PCR products were analysed by DNA-DNA hybridization. Sensitivity was enhanced by addition of a carrier to the samples before treatment and enabled detection of as few as 1 to 10 bacteria. Specific products were obtained only with the lyme disease agents, but not with other spirochetes or unrelated bacteria.
B. burgdorferi sensu lato was detected in cerebrospinal fluid (CSF) from 11 out of 45 patients with confirmed Lyme neuroborreliosis. In a prospective study, 20 out of 315 CSF samples from potential patients were PCR-positive. Forty uninfected patients were PCR-negative.
Un test d'amplification génique (PCR) a été développé pour la détection de
Borrelia burgdorferi sensu lato, agents de la maladie de Lyme. Un fragment de 333 bp provenant du palsmide circulaire de 30 kb de
B. burgdorferi B31 a été amplifié et les produits d'amplification analysis analysis par hybridation ADN/ADN. L'addition d'un entraîneur aux échantillons avant traitement a permis d'augmenter la sen sibilité Des produits spécifiques ont été obtenus uniquement avec les agents de la maladie de Lyme et pas avec d'autres spirochètes ou d'autres bactéries plus éloighées.
B. burgdorferi sensu lato a été détectée dans le liquide céphalorachidien (LCR) de ll patients sur 45 dont la neuroborréliose de Lyme avait été confirmée. Dans une étude prospective, 20 des 315 énhantillons de LCR étaient PCR
+; 40 patients non infectés étaient PCR
−.</description><identifier>ISSN: 0923-2508</identifier><identifier>EISSN: 1769-7123</identifier><identifier>DOI: 10.1016/0923-2508(93)90046-5</identifier><identifier>PMID: 8210678</identifier><language>eng</language><publisher>Paris: Elsevier SAS</publisher><subject>Bacterial diseases ; BACTERIOSE ; BACTERIOSES ; BACTERIOSIS ; Base Sequence - genetics ; Biological and medical sciences ; BORRELIA ; Borrelia burgdorferi ; Borrelia burgdorferi Group - genetics ; Borrelia burgdorferi Group - isolation & purification ; Borrelia infections ; CSF, Circular plasmid ; DIAGNOSIS ; DIAGNOSTIC ; DIAGNOSTICO ; Electrophoresis, Agar Gel ; GENERO HUMANO ; GENETIC ENGINEERING ; GENIE GENETIQUE ; GENRE HUMAIN ; Human bacterial diseases ; Humans ; In Vitro Techniques ; Infectious diseases ; INGENIERIA GENETICA ; IXODES ; Lyme Disease - cerebrospinal fluid ; MANKIND ; Medical sciences ; Molecular Sequence Data ; Nucleic Acid Hybridization ; PCR, Lyme disease, Neuroborreliosis, Borrelia burgdorferi ; PCR,, Neuroborréliose de Lyme, LCR, Plasmide circulaire ; Plasmids - genetics ; Polymerase Chain Reaction - methods ; Prospective Studies ; Tropical bacterial diseases ; VECTEUR DE MALADIE ; VECTORES ; VECTORS</subject><ispartof>Research in microbiology, 1993-03, Vol.144 (3), p.211-219</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c482t-cf216d94fae1487fec8508598a60cc78b8e0a582309b8ba4bac334c60a96a5083</citedby><cites>FETCH-LOGICAL-c482t-cf216d94fae1487fec8508598a60cc78b8e0a582309b8ba4bac334c60a96a5083</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0923250893900465$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4817574$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8210678$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Amouriaux, P.</creatorcontrib><creatorcontrib>Assous, M.</creatorcontrib><creatorcontrib>Margarita, D.</creatorcontrib><creatorcontrib>Baranton, G.</creatorcontrib><creatorcontrib>Girons, I.Saint</creatorcontrib><title>Polymerase chain reaction with the 30-kb circular plasmid of Borrelia burgdorferi B31 as a target for detection of the Lyme borreliosis agents in cerebrospinal fluid</title><title>Research in microbiology</title><addtitle>Res Microbiol</addtitle><description>The polymerase chain reaction (PCR) was developed for use in the detection of
Borrelia burgdorferi sensu lato, the Lyme disease agent. A 333-bp fragment of the 30-kbp circular plasmid from
Borrelia burgdorferi B31 was amplified and PCR products were analysed by DNA-DNA hybridization. Sensitivity was enhanced by addition of a carrier to the samples before treatment and enabled detection of as few as 1 to 10 bacteria. Specific products were obtained only with the lyme disease agents, but not with other spirochetes or unrelated bacteria.
B. burgdorferi sensu lato was detected in cerebrospinal fluid (CSF) from 11 out of 45 patients with confirmed Lyme neuroborreliosis. In a prospective study, 20 out of 315 CSF samples from potential patients were PCR-positive. Forty uninfected patients were PCR-negative.
Un test d'amplification génique (PCR) a été développé pour la détection de
Borrelia burgdorferi sensu lato, agents de la maladie de Lyme. Un fragment de 333 bp provenant du palsmide circulaire de 30 kb de
B. burgdorferi B31 a été amplifié et les produits d'amplification analysis analysis par hybridation ADN/ADN. L'addition d'un entraîneur aux échantillons avant traitement a permis d'augmenter la sen sibilité Des produits spécifiques ont été obtenus uniquement avec les agents de la maladie de Lyme et pas avec d'autres spirochètes ou d'autres bactéries plus éloighées.
B. burgdorferi sensu lato a été détectée dans le liquide céphalorachidien (LCR) de ll patients sur 45 dont la neuroborréliose de Lyme avait été confirmée. Dans une étude prospective, 20 des 315 énhantillons de LCR étaient PCR
+; 40 patients non infectés étaient PCR
−.</description><subject>Bacterial diseases</subject><subject>BACTERIOSE</subject><subject>BACTERIOSES</subject><subject>BACTERIOSIS</subject><subject>Base Sequence - genetics</subject><subject>Biological and medical sciences</subject><subject>BORRELIA</subject><subject>Borrelia burgdorferi</subject><subject>Borrelia burgdorferi Group - genetics</subject><subject>Borrelia burgdorferi Group - isolation & purification</subject><subject>Borrelia infections</subject><subject>CSF, Circular plasmid</subject><subject>DIAGNOSIS</subject><subject>DIAGNOSTIC</subject><subject>DIAGNOSTICO</subject><subject>Electrophoresis, Agar Gel</subject><subject>GENERO HUMANO</subject><subject>GENETIC ENGINEERING</subject><subject>GENIE GENETIQUE</subject><subject>GENRE HUMAIN</subject><subject>Human bacterial diseases</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Infectious diseases</subject><subject>INGENIERIA GENETICA</subject><subject>IXODES</subject><subject>Lyme Disease - cerebrospinal fluid</subject><subject>MANKIND</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Nucleic Acid Hybridization</subject><subject>PCR, Lyme disease, Neuroborreliosis, Borrelia burgdorferi</subject><subject>PCR,, Neuroborréliose de Lyme, LCR, Plasmide circulaire</subject><subject>Plasmids - genetics</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Prospective Studies</subject><subject>Tropical bacterial diseases</subject><subject>VECTEUR DE MALADIE</subject><subject>VECTORES</subject><subject>VECTORS</subject><issn>0923-2508</issn><issn>1769-7123</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc2O0zAUhS0EGkrhBRBIXiAEi4AdJ_7ZIDEjBpAqgRCsrRvnujWkccdOQPNAvCcuqbqElRf3O-fe40PIE85eccbla2ZqUdUt0y-MeGkYa2TV3iErrqSpFK_FXbI6I_fJg5y_M8ZbpZoLcqFrzqTSK_L7cxxu95ggI3U7CCNNCG4KcaS_wrSj0w6pYNWPjrqQ3DxAoocB8j70NHp6GVPCIQDt5rTtY_KYAr0UnEKmQCdIW5yoj4n2OOHiWlRHz01ZSrtFHnMo-BbHKdNygMOEXYr5EEYYqB_m0D8k9zwMGR-d3jX5dv3u69WHavPp_cert5vKNbqeKudrLnvTeEDeaOXR6ZK9NRokc07pTiODVteCmU530HTghGicZGAkFFKsyfPF95DizYx5svuQHQ4DjBjnbJVkta5r9V-QSyONLpvWpFlAVxLlhN4eUthDurWc2WON9tiRPXZkjbB_a7RtkT09-c_dHvuz6NRbmT87zSE7GHyC0YV8xhrNVauagj1eMA_RwjYV5PqLEUwofczwZhli-dGfAZPNLuDosA-plGX7GP595B_vpsK3</recordid><startdate>19930301</startdate><enddate>19930301</enddate><creator>Amouriaux, P.</creator><creator>Assous, M.</creator><creator>Margarita, D.</creator><creator>Baranton, G.</creator><creator>Girons, I.Saint</creator><general>Elsevier SAS</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19930301</creationdate><title>Polymerase chain reaction with the 30-kb circular plasmid of Borrelia burgdorferi B31 as a target for detection of the Lyme borreliosis agents in cerebrospinal fluid</title><author>Amouriaux, P. ; Assous, M. ; Margarita, D. ; Baranton, G. ; Girons, I.Saint</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c482t-cf216d94fae1487fec8508598a60cc78b8e0a582309b8ba4bac334c60a96a5083</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacterial diseases</topic><topic>BACTERIOSE</topic><topic>BACTERIOSES</topic><topic>BACTERIOSIS</topic><topic>Base Sequence - genetics</topic><topic>Biological and medical sciences</topic><topic>BORRELIA</topic><topic>Borrelia burgdorferi</topic><topic>Borrelia burgdorferi Group - genetics</topic><topic>Borrelia burgdorferi Group - isolation & purification</topic><topic>Borrelia infections</topic><topic>CSF, Circular plasmid</topic><topic>DIAGNOSIS</topic><topic>DIAGNOSTIC</topic><topic>DIAGNOSTICO</topic><topic>Electrophoresis, Agar Gel</topic><topic>GENERO HUMANO</topic><topic>GENETIC ENGINEERING</topic><topic>GENIE GENETIQUE</topic><topic>GENRE HUMAIN</topic><topic>Human bacterial diseases</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Infectious diseases</topic><topic>INGENIERIA GENETICA</topic><topic>IXODES</topic><topic>Lyme Disease - cerebrospinal fluid</topic><topic>MANKIND</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Nucleic Acid Hybridization</topic><topic>PCR, Lyme disease, Neuroborreliosis, Borrelia burgdorferi</topic><topic>PCR,, Neuroborréliose de Lyme, LCR, Plasmide circulaire</topic><topic>Plasmids - genetics</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Prospective Studies</topic><topic>Tropical bacterial diseases</topic><topic>VECTEUR DE MALADIE</topic><topic>VECTORES</topic><topic>VECTORS</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Amouriaux, P.</creatorcontrib><creatorcontrib>Assous, M.</creatorcontrib><creatorcontrib>Margarita, D.</creatorcontrib><creatorcontrib>Baranton, G.</creatorcontrib><creatorcontrib>Girons, I.Saint</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Research in microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Amouriaux, P.</au><au>Assous, M.</au><au>Margarita, D.</au><au>Baranton, G.</au><au>Girons, I.Saint</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Polymerase chain reaction with the 30-kb circular plasmid of Borrelia burgdorferi B31 as a target for detection of the Lyme borreliosis agents in cerebrospinal fluid</atitle><jtitle>Research in microbiology</jtitle><addtitle>Res Microbiol</addtitle><date>1993-03-01</date><risdate>1993</risdate><volume>144</volume><issue>3</issue><spage>211</spage><epage>219</epage><pages>211-219</pages><issn>0923-2508</issn><eissn>1769-7123</eissn><abstract>The polymerase chain reaction (PCR) was developed for use in the detection of
Borrelia burgdorferi sensu lato, the Lyme disease agent. A 333-bp fragment of the 30-kbp circular plasmid from
Borrelia burgdorferi B31 was amplified and PCR products were analysed by DNA-DNA hybridization. Sensitivity was enhanced by addition of a carrier to the samples before treatment and enabled detection of as few as 1 to 10 bacteria. Specific products were obtained only with the lyme disease agents, but not with other spirochetes or unrelated bacteria.
B. burgdorferi sensu lato was detected in cerebrospinal fluid (CSF) from 11 out of 45 patients with confirmed Lyme neuroborreliosis. In a prospective study, 20 out of 315 CSF samples from potential patients were PCR-positive. Forty uninfected patients were PCR-negative.
Un test d'amplification génique (PCR) a été développé pour la détection de
Borrelia burgdorferi sensu lato, agents de la maladie de Lyme. Un fragment de 333 bp provenant du palsmide circulaire de 30 kb de
B. burgdorferi B31 a été amplifié et les produits d'amplification analysis analysis par hybridation ADN/ADN. L'addition d'un entraîneur aux échantillons avant traitement a permis d'augmenter la sen sibilité Des produits spécifiques ont été obtenus uniquement avec les agents de la maladie de Lyme et pas avec d'autres spirochètes ou d'autres bactéries plus éloighées.
B. burgdorferi sensu lato a été détectée dans le liquide céphalorachidien (LCR) de ll patients sur 45 dont la neuroborréliose de Lyme avait été confirmée. Dans une étude prospective, 20 des 315 énhantillons de LCR étaient PCR
+; 40 patients non infectés étaient PCR
−.</abstract><cop>Paris</cop><pub>Elsevier SAS</pub><pmid>8210678</pmid><doi>10.1016/0923-2508(93)90046-5</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0923-2508 |
| ispartof | Research in microbiology, 1993-03, Vol.144 (3), p.211-219 |
| issn | 0923-2508 1769-7123 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_76028227 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Bacterial diseases BACTERIOSE BACTERIOSES BACTERIOSIS Base Sequence - genetics Biological and medical sciences BORRELIA Borrelia burgdorferi Borrelia burgdorferi Group - genetics Borrelia burgdorferi Group - isolation & purification Borrelia infections CSF, Circular plasmid DIAGNOSIS DIAGNOSTIC DIAGNOSTICO Electrophoresis, Agar Gel GENERO HUMANO GENETIC ENGINEERING GENIE GENETIQUE GENRE HUMAIN Human bacterial diseases Humans In Vitro Techniques Infectious diseases INGENIERIA GENETICA IXODES Lyme Disease - cerebrospinal fluid MANKIND Medical sciences Molecular Sequence Data Nucleic Acid Hybridization PCR, Lyme disease, Neuroborreliosis, Borrelia burgdorferi PCR,, Neuroborréliose de Lyme, LCR, Plasmide circulaire Plasmids - genetics Polymerase Chain Reaction - methods Prospective Studies Tropical bacterial diseases VECTEUR DE MALADIE VECTORES VECTORS |
| title | Polymerase chain reaction with the 30-kb circular plasmid of Borrelia burgdorferi B31 as a target for detection of the Lyme borreliosis agents in cerebrospinal fluid |
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