Synthesis and binding of new polyfluorinated aryl azides to .alpha.-chymotrypsin. New reagents for photoaffinity labeling

The preparation of several new, water-soluble, polyfluorinated aryl azide reagents for use in photoaffinity labeling studies is described. The ability of some of these reagents and their nonfluorinated controls to noncovalently bind to alpha-chrymotrypsin is reported. It is found that polyfluorinati...

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Veröffentlicht in:	Bioconjugate chemistry 1993-07, Vol.4 (4), p.256-261
Hauptverfasser:	Soundararajan, N, Liu, Shwu Huey, Soundararajan, S, Platz, M. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Affinity Labels - chemical synthesis Affinity Labels - chemistry Azides - chemistry Binding, Competitive Biological and medical sciences Chymotrypsin - antagonists & inhibitors Chymotrypsin - chemistry Fundamental and applied biological sciences. Psychology General aspects Hydrogen-Ion Concentration Indoles - pharmacology Instrumentation. Materials. Reagents. Research laboratory organization Kinetics Protein Binding Tyrosine - analogs & derivatives Tyrosine - chemistry
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Beschreibung
Zusammenfassung:	The preparation of several new, water-soluble, polyfluorinated aryl azide reagents for use in photoaffinity labeling studies is described. The ability of some of these reagents and their nonfluorinated controls to noncovalently bind to alpha-chrymotrypsin is reported. It is found that polyfluorination does not interfere with ligand binding.
ISSN:	1043-1802 1520-4812
DOI:	10.1021/bc00022a002