Bacitracin production by a new strain of Bacillus subtilis, extraction, purification, and characterization

A new strain of Bacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth of Micrococcus flavus. The production of the antibiotic in culture medium followed to extraction with n-butanol, thin layer chromatography, and microbiological tests...

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Veröffentlicht in:	Applied biochemistry and biotechnology 1993-07, Vol.42 (1), p.1-7
Hauptverfasser:	AZEVEDO, E. C, RIOS, E. M, FUKUSHIMA, K, CAMPOS-TAKAKI, G. M
Format:	Artikel
Sprache:	eng
Schlagworte:	1-Butanol Antibiotics Bacillus subtilis Bacillus subtilis - metabolism Bacitracin - biosynthesis Bacitracin - chemistry Bacitracin - isolation & purification Bacitracin - pharmacology Biological and medical sciences Biotechnology Butanols - chemistry Chromatography, High Pressure Liquid Chromatography, Thin Layer Culture Media Fundamental and applied biological sciences. Psychology Health. Pharmaceutical industry Industrial applications and implications. Economical aspects Microbial Sensitivity Tests Micrococcus - drug effects Micrococcus - growth & development Micrococcus flavus Production of active biomolecules
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Beschreibung
Zusammenfassung:	A new strain of Bacillus subtilis C 126 was isolated from sugar cane fermentation and produced an antibiotic that inhibited the growth of Micrococcus flavus. The production of the antibiotic in culture medium followed to extraction with n-butanol, thin layer chromatography, and microbiological tests indicated that a polypeptide antibiotic was produced. The fraction obtained by Sephadex G-25 column and analyzed by HPLC indicated that bacitracin complex was produced.
ISSN:	0273-2289 1559-0291
DOI:	10.1007/BF02788897