The use of colloidal gold for screening monoclonal antibodies to cell surface antigens

An immunogold method in Terasaki plates is described which allows accurate and sensitive visualization of the binding of monoclonal antibodies to cell surface antigens and is suitable for large scale screening. Monolayers of fixed cells are prepared in the wells. The binding of monoclonal antibodies...

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Veröffentlicht in:	Journal of immunological methods 1985-02, Vol.76 (2), p.211-222
Hauptverfasser:	Coppe, Ph, Letesson, J.-J., Saint-Guillain, M., Leloup, R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - immunology Antibody Specificity Antigens, Surface - analysis Biological and medical sciences cell surface antigens colloidal gold Colloids Fundamental and applied biological sciences. Psychology Fundamental immunology Gold Mice Microscopy, Electron Molecular immunology monoclonal antibodies Staphylococcal Protein A Techniques
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Beschreibung
Zusammenfassung:	An immunogold method in Terasaki plates is described which allows accurate and sensitive visualization of the binding of monoclonal antibodies to cell surface antigens and is suitable for large scale screening. Monolayers of fixed cells are prepared in the wells. The binding of monoclonal antibodies is detected by a protein A gold complex. The cell-bound gold can be visualized by either optical or transmission electron microscopy. The results obtained with various monoclonal antibodies are presented.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(85)90297-2