Enzyme amplification — A general method applied to provide an immunoassisted assay for placental alkaline phosphatase

A method is described in which enzyme amplification is used for the quantitative determination of placental alkaline phosphatase. In this particular application, placental alkaline phosphatase is captured on a solid surface by means of a monoclonal antibody. The enzyme is then determined by dephosph...

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Veröffentlicht in:	Journal of immunological methods 1985-02, Vol.76 (2), p.389-393
1. Verfasser:	Self, Colin H.
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - analysis Alkaline Phosphatase - immunology Antibodies, Monoclonal Biological and medical sciences enzyme amplification enzyme immunoassay Female Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Molecular immunology NAD - analysis NADP - metabolism Placenta - enzymology placental alkaline phosphatase Pregnancy Techniques
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Beschreibung
Zusammenfassung:	A method is described in which enzyme amplification is used for the quantitative determination of placental alkaline phosphatase. In this particular application, placental alkaline phosphatase is captured on a solid surface by means of a monoclonal antibody. The enzyme is then determined by dephosphorylation of NADP to NAD which activates a strictly NAD-dependent redox cycle leading to the formation of a coloured product.
ISSN:	0022-1759 1872-7905
DOI:	10.1016/0022-1759(85)90316-3