Rapid isolation of genes from bacterial lambda libraries by direct polymerase chain reaction screening

A method for the direct screening of bacterial lambda libraries by polymerase chain reaction technology has been developed. This technique permits the identification and isolation of specific DNA sequences without the need for any filter hybridisation or radioactive probing. This strategy has been u...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	FEMS microbiology letters 1993-08, Vol.112 (1), p.49-54
Hauptverfasser:	Griffin, H.G, I'Anson, K.J, Gasson, M.J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Bacteria - genetics Bacteriology Bacteriophage lambda - genetics Biological and medical sciences Cloning, Molecular Conserved Sequence DNA libraries Fundamental and applied biological sciences. Psychology Gene Library genes Genes, Bacterial Genetics Humans isolation L-Lactate Dehydrogenase - genetics Lactate dehydrogenase Lactic acid bacteria Lactococcus lactis Lactococcus lactis - enzymology Lactococcus lactis - genetics Library screening Microbiology Molecular Sequence Data phage lambda Polymerase chain reaction Polymerase Chain Reaction - methods rapid methods screening Sequence Homology, Amino Acid Species Specificity
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	A method for the direct screening of bacterial lambda libraries by polymerase chain reaction technology has been developed. This technique permits the identification and isolation of specific DNA sequences without the need for any filter hybridisation or radioactive probing. This strategy has been used to isolate a gene encoding lactate dehydrogenase from a Lactococcus lactis lambda library.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1993.tb06422.x