Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles: Interaction with cytoskeletal protein - vinculin
A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact spe...
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| Veröffentlicht in: | FEBS letters 1985-03, Vol.182 (1), p.67-72 |
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| creator | Koteliansky, V.E. Gneushev, G.N. Belkin, A.M. |
| description | A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinculin immobilized on polysterene surface. It is suggested that this 175-kDa protein may be involved in physical connection between microfilaments and cell membrane.
175-kDa membrane protein
Vinculin
Smooth muscle
Cardiac muscle
Cytoskeleton-membrane interaction |
| doi_str_mv | 10.1016/0014-5793(85)81155-8 |
| format | Article |
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175-kDa membrane protein
Vinculin
Smooth muscle
Cardiac muscle
Cytoskeleton-membrane interaction</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(85)81155-8</identifier><identifier>PMID: 3918890</identifier><identifier>CODEN: FEBLAL</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Cell membranes. Ionic channels. Membrane pores ; Cell structures and functions ; Chickens ; Electrophoresis, Polyacrylamide Gel ; Fluorescent Antibody Technique ; Fundamental and applied biological sciences. Psychology ; Gizzard, Avian - analysis ; Immunodiffusion ; membrane proteins ; Membrane Proteins - isolation & purification ; Membrane Proteins - metabolism ; Molecular and cellular biology ; Muscle Proteins - metabolism ; Muscle, Smooth - analysis ; muscles ; Myocardium - analysis ; Vinculin</subject><ispartof>FEBS letters, 1985-03, Vol.182 (1), p.67-72</ispartof><rights>1985</rights><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c281t-167dcea882dbae4142044184eb917d7f94c8e47513d79b43c32a073c355942803</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0014-5793(85)81155-8$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8454345$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3918890$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Koteliansky, V.E.</creatorcontrib><creatorcontrib>Gneushev, G.N.</creatorcontrib><creatorcontrib>Belkin, A.M.</creatorcontrib><title>Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles: Interaction with cytoskeletal protein - vinculin</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinculin immobilized on polysterene surface. It is suggested that this 175-kDa protein may be involved in physical connection between microfilaments and cell membrane.
175-kDa membrane protein
Vinculin
Smooth muscle
Cardiac muscle
Cytoskeleton-membrane interaction</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell membranes. Ionic channels. Membrane pores</subject><subject>Cell structures and functions</subject><subject>Chickens</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Fluorescent Antibody Technique</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gizzard, Avian - analysis</subject><subject>Immunodiffusion</subject><subject>membrane proteins</subject><subject>Membrane Proteins - isolation & purification</subject><subject>Membrane Proteins - metabolism</subject><subject>Molecular and cellular biology</subject><subject>Muscle Proteins - metabolism</subject><subject>Muscle, Smooth - analysis</subject><subject>muscles</subject><subject>Myocardium - analysis</subject><subject>Vinculin</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvFSEQgInR1NfqP9CEgzGauAoLPMCDiam1NmmiBz0TFmYjdhcqsG3qb-iPltf3fEd7mpD5ZpiZD6FnlLylhK7fEUJ5J6Rmr5R4rSgVolMP0IoqyTrG1-ohWu2Rx-iwlF-kvRXVB-iAaaqUJit0-23JYQzO1pAiTiO2mErRXXyyeIZ5yDYCvsypQohvcKgFT8nZKfzZ8iHiMqdUf2IbPXY2-2AdnpfiJijv8VmskK27Q69Do9xNTeUCJqh2-tcWd_gqRLdMIT5Bj0Y7FXi6i0fox-eT78dfuvOvp2fHH8871ytaO7qW3oFVqveDBU55TzinisOgqfRy1Nwp4FJQ5qUeOHOst0S2IITmvSLsCL3c9m0j_F6gVDOH4mCa2rZpKUYKrQXp-b3g5mvKdd9AvgVdTqVkGM1lDrPNN4YSs7FlNirMRoVRwtzZMqqVPd_1X4YZ_L5op6flX-zytrSzj02HC2WPKS4446JhH7YYtKNdBcimuADRgQ8ZXDU-hf_P8ReYCLDv</recordid><startdate>19850311</startdate><enddate>19850311</enddate><creator>Koteliansky, V.E.</creator><creator>Gneushev, G.N.</creator><creator>Belkin, A.M.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19850311</creationdate><title>Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles: Interaction with cytoskeletal protein - vinculin</title><author>Koteliansky, V.E. ; Gneushev, G.N. ; Belkin, A.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c281t-167dcea882dbae4142044184eb917d7f94c8e47513d79b43c32a073c355942803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cell membranes. Ionic channels. Membrane pores</topic><topic>Cell structures and functions</topic><topic>Chickens</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Fluorescent Antibody Technique</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gizzard, Avian - analysis</topic><topic>Immunodiffusion</topic><topic>membrane proteins</topic><topic>Membrane Proteins - isolation & purification</topic><topic>Membrane Proteins - metabolism</topic><topic>Molecular and cellular biology</topic><topic>Muscle Proteins - metabolism</topic><topic>Muscle, Smooth - analysis</topic><topic>muscles</topic><topic>Myocardium - analysis</topic><topic>Vinculin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Koteliansky, V.E.</creatorcontrib><creatorcontrib>Gneushev, G.N.</creatorcontrib><creatorcontrib>Belkin, A.M.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Koteliansky, V.E.</au><au>Gneushev, G.N.</au><au>Belkin, A.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles: Interaction with cytoskeletal protein - vinculin</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1985-03-11</date><risdate>1985</risdate><volume>182</volume><issue>1</issue><spage>67</spage><epage>72</epage><pages>67-72</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinculin immobilized on polysterene surface. It is suggested that this 175-kDa protein may be involved in physical connection between microfilaments and cell membrane.
175-kDa membrane protein
Vinculin
Smooth muscle
Cardiac muscle
Cytoskeleton-membrane interaction</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>3918890</pmid><doi>10.1016/0014-5793(85)81155-8</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1985-03, Vol.182 (1), p.67-72 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75995024 |
| source | MEDLINE; Elsevier ScienceDirect Journals; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Biological and medical sciences Cell membranes. Ionic channels. Membrane pores Cell structures and functions Chickens Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gizzard, Avian - analysis Immunodiffusion membrane proteins Membrane Proteins - isolation & purification Membrane Proteins - metabolism Molecular and cellular biology Muscle Proteins - metabolism Muscle, Smooth - analysis muscles Myocardium - analysis Vinculin |
| title | Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles: Interaction with cytoskeletal protein - vinculin |
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