Purification of a 175-kDa membrane protein, its localization in smooth and cardiac muscles: Interaction with cytoskeletal protein - vinculin

A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact spe...

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Veröffentlicht in:	FEBS letters 1985-03, Vol.182 (1), p.67-72
Hauptverfasser:	Koteliansky, V.E., Gneushev, G.N., Belkin, A.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Cell membranes. Ionic channels. Membrane pores Cell structures and functions Chickens Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Fundamental and applied biological sciences. Psychology Gizzard, Avian - analysis Immunodiffusion membrane proteins Membrane Proteins - isolation & purification Membrane Proteins - metabolism Molecular and cellular biology Muscle Proteins - metabolism Muscle, Smooth - analysis muscles Myocardium - analysis Vinculin
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Beschreibung
Zusammenfassung:	A new 175-kDa membrane protein was isolated from chicken gizzard smooth muscle. Antibodies to 175-kDa protein were used for localization of this protein in smooth and cardiac muscles. In both types of muscle 175-kDa protein was localized near plasma membrane. 175-kDa protein was able to interact specifically with vinculin immobilized on polysterene surface. It is suggested that this 175-kDa protein may be involved in physical connection between microfilaments and cell membrane. 175-kDa membrane protein Vinculin Smooth muscle Cardiac muscle Cytoskeleton-membrane interaction
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(85)81155-8