Dark-field optical coherence microscopy

Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light originating from the sample generates the tomogram...

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Veröffentlicht in:Optics letters 2010-10, Vol.35 (20), p.3489-3491
Hauptverfasser: VILLIGER, Martin, PACHE, Christophe, LASSER, Theo
Format: Artikel
Sprache:eng
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Zusammenfassung:Dark-field illumination is known to enhance scattering contrast in optical microscopy. We combined this concept with Fourier domain optical coherence microscopy (OCM). The detection and illumination paths are decoupled, and only the scattered light originating from the sample generates the tomogram signal, whereas any specular reflection is highly suppressed. We analyze and discuss this dark-field OCM concept and present its superior imaging quality on live cell samples.
ISSN:0146-9592
1539-4794
DOI:10.1364/OL.35.003489