Selective determination of aloin in different matrices by HPTLC densitometry in fluorescence mode

A novel method based on the fluorescence excited solely on aloin by a H 3BO 3 derivatizing procedure, allowed its rapid and selective determination among the co-occurring components in a variety of complex matrices as several Aloes dried extracts and related commercial products. HPTLC LiChrospher silica gel 60 F254S, 20 cm × 10 cm, plates with ethyl formate: CH 3OH:H 2O (100:14.5:10, v/v) as the mobile phase were used. Densitometric determinations were performed in fluorescence mode, exciting wavelength 365 nm, optical filter K540 after derivatization with H 3BO 3. The method was validated giving rise to a dependable and high throughput procedure well suited to routine application. Aloin was quantified in the range of 110–330 ng with RSD of repeatability and intermediate precision not exceeding 2.3% and accuracy inside the acceptance limits.