Assay of Brain Calmodulin Levels Using High‐Performance Liquid Chromatography

: A simple, sensitive, and efficient HPLC method for the determination of calmodulin levels in brain tissue extracts is described. The assay is linear with respect to both calmodulin and protein concentrations. The specificity and validity of this assay for calmodulin is demonstrated by parallel rad...

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Veröffentlicht in:	Journal of neurochemistry 1985-04, Vol.44 (4), p.1111-1115
Hauptverfasser:	Loullis, Costas C., Antonian, Lida, Rauh, Charles E., Fliegner, Karsten, Coupet, Joseph, Lippa, Arnold S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biochemistry and metabolism Biological and medical sciences Brain Chemistry Calmodulin Calmodulin - analysis Central nervous system Chromatography, High Pressure Liquid Fundamental and applied biological sciences. Psychology HPLC Hypothalamus - analysis Male Radioimmunoassay Rats Rats, Inbred Strains Tissue Distribution Vertebrates: nervous system and sense organs
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Beschreibung
Zusammenfassung:	: A simple, sensitive, and efficient HPLC method for the determination of calmodulin levels in brain tissue extracts is described. The assay is linear with respect to both calmodulin and protein concentrations. The specificity and validity of this assay for calmodulin is demonstrated by parallel radioimmunoassay determinations which give equivalent results. Determination of calmodulin levels in various brain regions revealed a high concentration of this protein in the hypothalamus, by comparison to other areas examined.
ISSN:	0022-3042 1471-4159
DOI:	10.1111/j.1471-4159.1985.tb08732.x