Isolation of inhibin from bovine follicular fluid

Bovine follicular fluid was used as a source for the isolation of gonadal inhibin, the activity of which was monitored by the dose dependent suppression of the FSH content of cultured pituitary cells. The procedures presented result in over 3000-fold purification of the starting material and the pur...

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Veröffentlicht in:	Biochemical and biophysical research communications 1985-01, Vol.126 (1), p.220-226
Hauptverfasser:	Robertson, D.M., Foulds, L.M., Leversha, L., Morgan, F.J., Hearn, M.T.W., Burger, H.G., Wettenhall, R.E.H., de Kretser, D.M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Applied sciences Biological Assay Cattle Cells, Cultured Chromatography, Gel Chromatography, High Pressure Liquid Electrophoresis, Polyacrylamide Gel Exact sciences and technology Female Follicle Stimulating Hormone - metabolism follicles inhibin Inhibins - isolation & purification Molecular Weight Other techniques and industries Ovarian Follicle - metabolism Pituitary Gland - drug effects
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Zusammenfassung:	Bovine follicular fluid was used as a source for the isolation of gonadal inhibin, the activity of which was monitored by the dose dependent suppression of the FSH content of cultured pituitary cells. The procedures presented result in over 3000-fold purification of the starting material and the purified inhibin has an apparent molecular weight of 56000. The purified inhibin can be dissociated under reducing conditions into two subunits with molecular weights of 44000 and 14000 daltons.
ISSN:	0006-291X 1090-2104
DOI:	10.1016/0006-291X(85)90594-7