Application of an integrated rate equation to the inactivation of catalase

A feature of catalase that has received scant attention in recent years and that may have physiological significance is the peroxide-dependent inactivation of the enzyme. In this article we show how to obtain the second-order rate constant for the inactivation reaction by fitting the reaction progre...

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Veröffentlicht in:	Free radical biology & medicine 1993-08, Vol.15 (2), p.223-226
Hauptverfasser:	Feuers, Ritchie J., Pattillo, Francis M., Osborn, Criston K., Adams, Karen L., DeLuca, Donald, Grady Smith, W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Catalase Catalase - antagonists & inhibitors Catalase - metabolism Cattle Enzyme inactivation Enzymes and enzyme inhibitors Free radicals Fundamental and applied biological sciences. Psychology Integrated rate equation Kinetics Liver - enzymology Mathematics Oxidoreductases
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Zusammenfassung:	A feature of catalase that has received scant attention in recent years and that may have physiological significance is the peroxide-dependent inactivation of the enzyme. In this article we show how to obtain the second-order rate constant for the inactivation reaction by fitting the reaction progress curve to an integrated rate equation. This method will simplify quantitation of the inactivation and reactivation of catalase. These measurements in tissues with altered metabolic states may reveal new information about the role of catalase in nutrition, aging, and pathology.
ISSN:	0891-5849 1873-4596
DOI:	10.1016/0891-5849(93)90063-Z