Cloning and functional expression of a human parathyroid hormone receptor

We have cloned a human receptor for parathyroid hormone from a kidney complementary DNA library. The deduced sequence of 593 amino acids shows high homology to the previously cloned receptors from opossum and rat. Expressed in COS-1 cells, the human receptor binds to parathyroid hormone-(1–38) with...

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Veröffentlicht in:	European journal of pharmacology 1993-07, Vol.246 (2), p.149-155
Hauptverfasser:	Schneider, Helmut, Feyen, Jean H.M., Seuwen, Klaus, Rao Movva, N.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenylyl Cyclases - metabolism Animals Base Sequence Biological and medical sciences Blotting, Northern cDNA sequence Cell receptors Cell structures and functions Cells, Cultured Cloning, Molecular DNA - biosynthesis Functional expression Fundamental and applied biological sciences. Psychology Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors Humans Kidney - enzymology Kidney - metabolism Molecular and cellular biology Molecular Sequence Data Parathyroid Hormone - metabolism Parathyroid hormone receptor Polymerase Chain Reaction Rats Receptors, Cell Surface - metabolism Receptors, Parathyroid Hormone
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Zusammenfassung:	We have cloned a human receptor for parathyroid hormone from a kidney complementary DNA library. The deduced sequence of 593 amino acids shows high homology to the previously cloned receptors from opossum and rat. Expressed in COS-1 cells, the human receptor binds to parathyroid hormone-(1–38) with high affinity (pK D = 8.5) and is functionally coupled to adenylate cyclase (pEC 50 = 9.4). At high concentrations of agonist, the receptor also activates phosphoinositide turnover.
ISSN:	0922-4106 0014-2999
DOI:	10.1016/0922-4106(93)90092-N