Paired STSs amplified from radiation hybrids, and from associated YACs, identify highly polymorphic loci flanking the ataxia telangiectasia locus on chromosome 11q22–23
The high resolution mapping of the ataxia telangiectasia (A-T) locus on chromosome 11q22–23 requires the generation of new polymorphic markers specifically within the segment of 11q22–23 to which the locus has been assigned. We have made use of a library of Alu-PCR clones, amplified from a radiation...
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Veröffentlicht in: | Human molecular genetics 1993-07, Vol.2 (7), p.969-974 |
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Sprache: | eng |
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Zusammenfassung: | The high resolution mapping of the ataxia telangiectasia (A-T) locus on chromosome 11q22–23 requires the generation of new polymorphic markers specifically within the segment of 11q22–23 to which the locus has been assigned. We have made use of a library of Alu-PCR clones, amplified from a radiation reduced somatic cell hybrid containing the relevant chromosome 11 segment, to generate sequence tagged sites (STS) within the 11q22–23 region and have used YAC clones to extend the loci identified by these STSs. The identification of paired polymorphisms (from Alu-PCR and the associated YAC derived clone), which are physically linked, but which show minimal linkage disequilibrium, provides a highly informative haplotype for use in genetic linkage analysis in A-T famllies. We describe the characterisation of 2 such polymorphic locl, D11S535 and D11S611, which map between existing flanking markers, and which provide additional information on the location of the major A-T locus. |
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ISSN: | 0964-6906 1460-2083 |
DOI: | 10.1093/hmg/2.7.969 |