Inhibition of the activity of restriction endonucleases by spermidine and spermine

Physiological concentrations (0.5–2.0 mM) of spermidine and spermine were observed to inhibit the digestion in vitro of plasmid pJDB 207 by the restriction endonucleases BamHI (EC 3.1.23.6), EcoRI (EC 3.2.23.13), HindIII (EC 3.1.23.20), HpaI (EC 3.1.23.23) and PstI (EC 3.1.23.31). The polyamines pro...

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Veröffentlicht in:	FEBS letters 1985, Vol.179 (1), p.17-20
Hauptverfasser:	Kuosmanen, Malla, Pösö, Hannu
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Biological and medical sciences DNA Restriction Enzymes - antagonists & inhibitors Endonuclease Enzymes and enzyme inhibitors Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Hydrolases Kinetics Plasmid digestion inhibition Plasmids Polyamine Putrescine - pharmacology Spermidine - pharmacology Spermine - pharmacology
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Zusammenfassung:	Physiological concentrations (0.5–2.0 mM) of spermidine and spermine were observed to inhibit the digestion in vitro of plasmid pJDB 207 by the restriction endonucleases BamHI (EC 3.1.23.6), EcoRI (EC 3.2.23.13), HindIII (EC 3.1.23.20), HpaI (EC 3.1.23.23) and PstI (EC 3.1.23.31). The polyamines protected all the tested restriction sequences of DNA, since the activity of all endonucleases used was strongly inhibited. These results show the need for caution when using polyamines as experimental tools for recombinant DNA chemistry.
ISSN:	0014-5793 1873-3468
DOI:	10.1016/0014-5793(85)80181-2