Cell Swelling and the sensitivity of autophagic proteolysis to inhibition by amino acids in isolated rat hepatocytes
In the isolated perfused rat liver, autophagic proteolysis is inhibited by hypo‐osmotic perfusion media [Häussinger, D., Hallbrucker, C., vom Dahl, S., Lang, F. & Gerok, W. (1990) Biochem. J. 272, 239–242]. Here we report that in isolated hepatocytes, incubated in the absence of amino acids to e...
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creator | MEIJER, Alfred J. GUSTAFSON, Lori A. LUIKEN, Joost J. F. P. BLOMMAART, Pietjan J. E. CARO, L. Heleen P. WOERKOM, George M. SPRONK, Claire BOON, Louis |
description | In the isolated perfused rat liver, autophagic proteolysis is inhibited by hypo‐osmotic perfusion media [Häussinger, D., Hallbrucker, C., vom Dahl, S., Lang, F. & Gerok, W. (1990) Biochem. J. 272, 239–242]. Here we report that in isolated hepatocytes, incubated in the absence of amino acids to ensure maximal proteolytic flux, proteolysis was not inhibited by hypo‐osmolarity while the synthesis of glycogen from glucose, a process known to be very sensitive to changes in cell volume [Baquet, A., Hue, L., Meijer, A. J., van Woerkom, G. M. & Plomp, P. J. A. M. (1990) J. Biol. Chem. 265, 955–959], was stimulated under identical conditions. However, in isolated hepatocytes, hypo‐osmolarity increased the sensitivity of autophagic proteolysis to inhibition by low concentrations of amino acids. The anti‐proteolytic effect of hypo‐osmolarity in our experiments was not due to stimulation of amino‐acid transport into the hepatocytes: neither the consumption of most amino acids, nor the rate of urea synthesis was appreciably affected by hypo‐osmotic incubation conditions. In the course of these studies we also found that hypo‐osmolarity increased the affinity of protein synthesis for amino acids.
In the presence of amino acids the intracellular level of ATP was not much affected. However, because of cell swelling under these conditions the intracelluar concentration of ATP decreased. It is proposed that a small part of the inhibition of proteolysis by amino acids may be due to this fall in ATP concentration. |
doi_str_mv | 10.1111/j.1432-1033.1993.tb18053.x |
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In the presence of amino acids the intracellular level of ATP was not much affected. However, because of cell swelling under these conditions the intracelluar concentration of ATP decreased. It is proposed that a small part of the inhibition of proteolysis by amino acids may be due to this fall in ATP concentration.</description><identifier>ISSN: 0014-2956</identifier><identifier>EISSN: 1432-1033</identifier><identifier>DOI: 10.1111/j.1432-1033.1993.tb18053.x</identifier><identifier>PMID: 8344312</identifier><identifier>CODEN: EJBCAI</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adenosine Triphosphate - metabolism ; Amino Acids - metabolism ; Amino Acids - pharmacology ; Animals ; Autophagy - drug effects ; Biological and medical sciences ; Cell metabolism, cell oxidation ; Cell physiology ; Fundamental and applied biological sciences. Psychology ; Glycogen - biosynthesis ; Liver - cytology ; Liver - drug effects ; Liver - metabolism ; Male ; Molecular and cellular biology ; Osmolar Concentration ; Proteins - metabolism ; Rats ; Rats, Wistar ; Sodium Chloride - pharmacology</subject><ispartof>European journal of biochemistry, 1993-07, Vol.215 (2), p.449-454</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4519-df10cc2cf0cc7424e2665b84095e225cad365b932dad1be7dc7f85f782563dc63</citedby><cites>FETCH-LOGICAL-c4519-df10cc2cf0cc7424e2665b84095e225cad365b932dad1be7dc7f85f782563dc63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4862079$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8344312$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MEIJER, Alfred J.</creatorcontrib><creatorcontrib>GUSTAFSON, Lori A.</creatorcontrib><creatorcontrib>LUIKEN, Joost J. F. P.</creatorcontrib><creatorcontrib>BLOMMAART, Pietjan J. E.</creatorcontrib><creatorcontrib>CARO, L. Heleen P.</creatorcontrib><creatorcontrib>WOERKOM, George M.</creatorcontrib><creatorcontrib>SPRONK, Claire</creatorcontrib><creatorcontrib>BOON, Louis</creatorcontrib><title>Cell Swelling and the sensitivity of autophagic proteolysis to inhibition by amino acids in isolated rat hepatocytes</title><title>European journal of biochemistry</title><addtitle>Eur J Biochem</addtitle><description>In the isolated perfused rat liver, autophagic proteolysis is inhibited by hypo‐osmotic perfusion media [Häussinger, D., Hallbrucker, C., vom Dahl, S., Lang, F. & Gerok, W. (1990) Biochem. J. 272, 239–242]. Here we report that in isolated hepatocytes, incubated in the absence of amino acids to ensure maximal proteolytic flux, proteolysis was not inhibited by hypo‐osmolarity while the synthesis of glycogen from glucose, a process known to be very sensitive to changes in cell volume [Baquet, A., Hue, L., Meijer, A. J., van Woerkom, G. M. & Plomp, P. J. A. M. (1990) J. Biol. Chem. 265, 955–959], was stimulated under identical conditions. However, in isolated hepatocytes, hypo‐osmolarity increased the sensitivity of autophagic proteolysis to inhibition by low concentrations of amino acids. The anti‐proteolytic effect of hypo‐osmolarity in our experiments was not due to stimulation of amino‐acid transport into the hepatocytes: neither the consumption of most amino acids, nor the rate of urea synthesis was appreciably affected by hypo‐osmotic incubation conditions. In the course of these studies we also found that hypo‐osmolarity increased the affinity of protein synthesis for amino acids.
In the presence of amino acids the intracellular level of ATP was not much affected. However, because of cell swelling under these conditions the intracelluar concentration of ATP decreased. It is proposed that a small part of the inhibition of proteolysis by amino acids may be due to this fall in ATP concentration.</description><subject>Adenosine Triphosphate - metabolism</subject><subject>Amino Acids - metabolism</subject><subject>Amino Acids - pharmacology</subject><subject>Animals</subject><subject>Autophagy - drug effects</subject><subject>Biological and medical sciences</subject><subject>Cell metabolism, cell oxidation</subject><subject>Cell physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Glycogen - biosynthesis</subject><subject>Liver - cytology</subject><subject>Liver - drug effects</subject><subject>Liver - metabolism</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>Osmolar Concentration</subject><subject>Proteins - metabolism</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Sodium Chloride - pharmacology</subject><issn>0014-2956</issn><issn>1432-1033</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkEtr3DAUhUVJSSdpf0JBlNCdXT1tq5vQDnkUAl2kXQtZkjMaPNbUV9PE_z4yY2YfLa4Q59x7jz6EvlBS0ny-bUsqOCso4bykSvEytbQhkpcv79DqJJ2hFSFUFEzJ6gO6ANgSQipV1efovOFCcMpWKK193-PH51zD8ITN4HDaeAx-gJDC_5AmHDtsDinuN-YpWLwfY_KxnyAAThGHYRPa7IwDbidsdmGI2NjgICs4QOxN8g6PJuGN35sU7ZQ8fETvO9OD_7Tcl-jv7c2f9X3x8Pvu1_rHQ2GFpKpwHSXWMtvlWgsmPKsq2TaCKOkZk9Y4nt-KM2ccbX3tbN01sqsbJivubMUv0dfj3Bz638FD0rsANn_VDD4eQNdSUZbHZeP3o9GOEWD0nd6PYWfGSVOiZ-R6q2eueuaqZ-R6Qa5fcvPnZcuh3Xl3al0YZ_1q0Q1Y03ejGWyAk000FSP1nOH6aHsOvZ_eEEDf3vx8FELxV5GeoFQ</recordid><startdate>19930715</startdate><enddate>19930715</enddate><creator>MEIJER, Alfred J.</creator><creator>GUSTAFSON, Lori A.</creator><creator>LUIKEN, Joost J. F. P.</creator><creator>BLOMMAART, Pietjan J. E.</creator><creator>CARO, L. Heleen P.</creator><creator>WOERKOM, George M.</creator><creator>SPRONK, Claire</creator><creator>BOON, Louis</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930715</creationdate><title>Cell Swelling and the sensitivity of autophagic proteolysis to inhibition by amino acids in isolated rat hepatocytes</title><author>MEIJER, Alfred J. ; GUSTAFSON, Lori A. ; LUIKEN, Joost J. F. P. ; BLOMMAART, Pietjan J. E. ; CARO, L. Heleen P. ; WOERKOM, George M. ; SPRONK, Claire ; BOON, Louis</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4519-df10cc2cf0cc7424e2665b84095e225cad365b932dad1be7dc7f85f782563dc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adenosine Triphosphate - metabolism</topic><topic>Amino Acids - metabolism</topic><topic>Amino Acids - pharmacology</topic><topic>Animals</topic><topic>Autophagy - drug effects</topic><topic>Biological and medical sciences</topic><topic>Cell metabolism, cell oxidation</topic><topic>Cell physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Glycogen - biosynthesis</topic><topic>Liver - cytology</topic><topic>Liver - drug effects</topic><topic>Liver - metabolism</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>Osmolar Concentration</topic><topic>Proteins - metabolism</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Sodium Chloride - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MEIJER, Alfred J.</creatorcontrib><creatorcontrib>GUSTAFSON, Lori A.</creatorcontrib><creatorcontrib>LUIKEN, Joost J. F. P.</creatorcontrib><creatorcontrib>BLOMMAART, Pietjan J. E.</creatorcontrib><creatorcontrib>CARO, L. Heleen P.</creatorcontrib><creatorcontrib>WOERKOM, George M.</creatorcontrib><creatorcontrib>SPRONK, Claire</creatorcontrib><creatorcontrib>BOON, Louis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MEIJER, Alfred J.</au><au>GUSTAFSON, Lori A.</au><au>LUIKEN, Joost J. F. P.</au><au>BLOMMAART, Pietjan J. E.</au><au>CARO, L. Heleen P.</au><au>WOERKOM, George M.</au><au>SPRONK, Claire</au><au>BOON, Louis</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cell Swelling and the sensitivity of autophagic proteolysis to inhibition by amino acids in isolated rat hepatocytes</atitle><jtitle>European journal of biochemistry</jtitle><addtitle>Eur J Biochem</addtitle><date>1993-07-15</date><risdate>1993</risdate><volume>215</volume><issue>2</issue><spage>449</spage><epage>454</epage><pages>449-454</pages><issn>0014-2956</issn><eissn>1432-1033</eissn><coden>EJBCAI</coden><abstract>In the isolated perfused rat liver, autophagic proteolysis is inhibited by hypo‐osmotic perfusion media [Häussinger, D., Hallbrucker, C., vom Dahl, S., Lang, F. & Gerok, W. (1990) Biochem. J. 272, 239–242]. Here we report that in isolated hepatocytes, incubated in the absence of amino acids to ensure maximal proteolytic flux, proteolysis was not inhibited by hypo‐osmolarity while the synthesis of glycogen from glucose, a process known to be very sensitive to changes in cell volume [Baquet, A., Hue, L., Meijer, A. J., van Woerkom, G. M. & Plomp, P. J. A. M. (1990) J. Biol. Chem. 265, 955–959], was stimulated under identical conditions. However, in isolated hepatocytes, hypo‐osmolarity increased the sensitivity of autophagic proteolysis to inhibition by low concentrations of amino acids. The anti‐proteolytic effect of hypo‐osmolarity in our experiments was not due to stimulation of amino‐acid transport into the hepatocytes: neither the consumption of most amino acids, nor the rate of urea synthesis was appreciably affected by hypo‐osmotic incubation conditions. In the course of these studies we also found that hypo‐osmolarity increased the affinity of protein synthesis for amino acids.
In the presence of amino acids the intracellular level of ATP was not much affected. However, because of cell swelling under these conditions the intracelluar concentration of ATP decreased. It is proposed that a small part of the inhibition of proteolysis by amino acids may be due to this fall in ATP concentration.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8344312</pmid><doi>10.1111/j.1432-1033.1993.tb18053.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Adenosine Triphosphate - metabolism Amino Acids - metabolism Amino Acids - pharmacology Animals Autophagy - drug effects Biological and medical sciences Cell metabolism, cell oxidation Cell physiology Fundamental and applied biological sciences. Psychology Glycogen - biosynthesis Liver - cytology Liver - drug effects Liver - metabolism Male Molecular and cellular biology Osmolar Concentration Proteins - metabolism Rats Rats, Wistar Sodium Chloride - pharmacology |
title | Cell Swelling and the sensitivity of autophagic proteolysis to inhibition by amino acids in isolated rat hepatocytes |
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