Cell Swelling and the sensitivity of autophagic proteolysis to inhibition by amino acids in isolated rat hepatocytes

In the isolated perfused rat liver, autophagic proteolysis is inhibited by hypo‐osmotic perfusion media [Häussinger, D., Hallbrucker, C., vom Dahl, S., Lang, F. & Gerok, W. (1990) Biochem. J. 272, 239–242]. Here we report that in isolated hepatocytes, incubated in the absence of amino acids to e...

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Veröffentlicht in:European journal of biochemistry 1993-07, Vol.215 (2), p.449-454
Hauptverfasser: MEIJER, Alfred J., GUSTAFSON, Lori A., LUIKEN, Joost J. F. P., BLOMMAART, Pietjan J. E., CARO, L. Heleen P., WOERKOM, George M., SPRONK, Claire, BOON, Louis
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container_issue 2
container_start_page 449
container_title European journal of biochemistry
container_volume 215
creator MEIJER, Alfred J.
GUSTAFSON, Lori A.
LUIKEN, Joost J. F. P.
BLOMMAART, Pietjan J. E.
CARO, L. Heleen P.
WOERKOM, George M.
SPRONK, Claire
BOON, Louis
description In the isolated perfused rat liver, autophagic proteolysis is inhibited by hypo‐osmotic perfusion media [Häussinger, D., Hallbrucker, C., vom Dahl, S., Lang, F. & Gerok, W. (1990) Biochem. J. 272, 239–242]. Here we report that in isolated hepatocytes, incubated in the absence of amino acids to ensure maximal proteolytic flux, proteolysis was not inhibited by hypo‐osmolarity while the synthesis of glycogen from glucose, a process known to be very sensitive to changes in cell volume [Baquet, A., Hue, L., Meijer, A. J., van Woerkom, G. M. & Plomp, P. J. A. M. (1990) J. Biol. Chem. 265, 955–959], was stimulated under identical conditions. However, in isolated hepatocytes, hypo‐osmolarity increased the sensitivity of autophagic proteolysis to inhibition by low concentrations of amino acids. The anti‐proteolytic effect of hypo‐osmolarity in our experiments was not due to stimulation of amino‐acid transport into the hepatocytes: neither the consumption of most amino acids, nor the rate of urea synthesis was appreciably affected by hypo‐osmotic incubation conditions. In the course of these studies we also found that hypo‐osmolarity increased the affinity of protein synthesis for amino acids. In the presence of amino acids the intracellular level of ATP was not much affected. However, because of cell swelling under these conditions the intracelluar concentration of ATP decreased. It is proposed that a small part of the inhibition of proteolysis by amino acids may be due to this fall in ATP concentration.
doi_str_mv 10.1111/j.1432-1033.1993.tb18053.x
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In the presence of amino acids the intracellular level of ATP was not much affected. However, because of cell swelling under these conditions the intracelluar concentration of ATP decreased. It is proposed that a small part of the inhibition of proteolysis by amino acids may be due to this fall in ATP concentration.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8344312</pmid><doi>10.1111/j.1432-1033.1993.tb18053.x</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
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ispartof European journal of biochemistry, 1993-07, Vol.215 (2), p.449-454
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1432-1033
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subjects Adenosine Triphosphate - metabolism
Amino Acids - metabolism
Amino Acids - pharmacology
Animals
Autophagy - drug effects
Biological and medical sciences
Cell metabolism, cell oxidation
Cell physiology
Fundamental and applied biological sciences. Psychology
Glycogen - biosynthesis
Liver - cytology
Liver - drug effects
Liver - metabolism
Male
Molecular and cellular biology
Osmolar Concentration
Proteins - metabolism
Rats
Rats, Wistar
Sodium Chloride - pharmacology
title Cell Swelling and the sensitivity of autophagic proteolysis to inhibition by amino acids in isolated rat hepatocytes
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