Endogenous factor activating Na,K-ATPase induced by blockade of adrenoceptors

It was established that the regulatory factor released from the effector cells under the blockade of postsynaptic α- and β-adrenoceptors by phentolamine and propranolol (0.5–1.0 μM) and activating noradrenergic uptake and synthesis [1, 2] increased the activity of Na,K-ATPase of isolated rat organs and partially purified Na, K-ATPase isolated from the rat brain. The regulatory factor is a protein with molecular mass 25–100 kDa, adsorbed on phenylsepharose CL-4B and eluated with 30 mM KC1. It consists of two active components with pI 5.1 and 5.9 which in admixture (1:1) activate Na,K-ATPase by 60%.