Determination of phosphoethanolamine in urine or in the presence of high taurine concentrations

Phosphoethanolamine forms a low-solubility lead salt, which may be precipitated from urine together with inorganic phosphate, thus separating taurine, urea, and other interfering substances of chromatographic determination. Fluorometric detection of phosphoethanolamine with o-phthaldialdehyde after...

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Veröffentlicht in:	Analytical biochemistry 1984-11, Vol.142 (2), p.511-514
Hauptverfasser:	Gundlach, G., Käbisch, A., Voegeli, R., Pralle, H., Hartenfels, S.
Format:	Artikel
Sprache:	eng
Schlagworte:	2-aminoethanolphosphate Analytical, structural and metabolic biochemistry Biological and medical sciences Chemical Precipitation chromatographic determination Chromatography, Ion Exchange Ethanolamines - urine Fundamental and applied biological sciences. Psychology Humans Hydrogen-Ion Concentration Lead lead-salt precipitation Lipids Other biological molecules phosphoethanolamine Solubility Spectrometry, Fluorescence taurine Taurine - urine
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Beschreibung
Zusammenfassung:	Phosphoethanolamine forms a low-solubility lead salt, which may be precipitated from urine together with inorganic phosphate, thus separating taurine, urea, and other interfering substances of chromatographic determination. Fluorometric detection of phosphoethanolamine with o-phthaldialdehyde after elution from a Dowex 50-X10 column enhances the specificity of the method once more. The method described is fast and easy to perform.
ISSN:	0003-2697 1096-0309
DOI:	10.1016/0003-2697(84)90497-4