Transformation of Zymomonas mobilis by a hybrid plasmid

The transformation of Zymomonas mobilis by plasmid DNA was achieved using a modification of the CaCl 2 method for Escherichia coli. The highest frequency of transformation obtained was 5 × 10 3 transformants/μg DNA. The success of the method depended upon the use of a plasmid which is a cointegrate...

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Veröffentlicht in:	Plasmid 1984-01, Vol.12 (3), p.211-214
Hauptverfasser:	Browne, G.M., Skotnicki, M.L., Goodman, A.E., Rogers, P.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Cloning, Molecular Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Genetic Vectors Methods. Procedures. Technologies Plasmids Pseudomonadaceae - genetics transformation Transformation, Genetic Vectors (cloning, transfer, expression). Insertion sequences and transposons Zymomonas mobilis
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Zusammenfassung:	The transformation of Zymomonas mobilis by plasmid DNA was achieved using a modification of the CaCl 2 method for Escherichia coli. The highest frequency of transformation obtained was 5 × 10 3 transformants/μg DNA. The success of the method depended upon the use of a plasmid which is a cointegrate between a Z. mobilis cryptic plasmid and an E. coli plasmid carrying two selectable drug resistance markers.
ISSN:	0147-619X 1095-9890
DOI:	10.1016/0147-619X(84)90047-7