Adhesion of Cerebral Endothelial Cells to Lymphocytes from Patients with Multiple Sclerosis

To investigate the factors regulating the entry of lymphocytes into the brain, we assessed the adhesion in vitro of 51Cr labelled lymphocytes from peripheral blood of patients with multiple sclerosis (MS) to human cerebral endothelial cells, and evaluated the effect on the adhesion of endothelium ac...

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Veröffentlicht in:Autoimmunity (Chur, Switzerland) Switzerland), 1993, Vol.14 (4), p.329-333
Hauptverfasser: Tsukada, Naoyuki, Matsuda, Masayuki, Miyagi, Koichi, Yanagisawa, Nobuo
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container_issue 4
container_start_page 329
container_title Autoimmunity (Chur, Switzerland)
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creator Tsukada, Naoyuki
Matsuda, Masayuki
Miyagi, Koichi
Yanagisawa, Nobuo
description To investigate the factors regulating the entry of lymphocytes into the brain, we assessed the adhesion in vitro of 51Cr labelled lymphocytes from peripheral blood of patients with multiple sclerosis (MS) to human cerebral endothelial cells, and evaluated the effect on the adhesion of endothelium activated by interferon-γ (IFN-γ). lipopolysaccharides (LPS), interleukin-1 (IL-1) and tumor necrosis factor (TNF). Patients with acute relapsing MS during an exacerbation showed significant increase in MNC adherence to cerebral. endothelial cells as compared with controls (p
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Patients with acute relapsing MS during an exacerbation showed significant increase in MNC adherence to cerebral. endothelial cells as compared with controls (p&lt;0.001). MNC adherence to cerebral endothelial cells activated by IFN-γ or LPS, was significantly increased as compared to the controls (p&lt;0.01). MNC adherence to endothelial cells was not blocked by antibodies against the intercellular adhesion molecule-1 (ICAM-1), but was blocked by lymphocyte function-associated antigen-1 (LFA-1). 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Patients with acute relapsing MS during an exacerbation showed significant increase in MNC adherence to cerebral. endothelial cells as compared with controls (p&lt;0.001). MNC adherence to cerebral endothelial cells activated by IFN-γ or LPS, was significantly increased as compared to the controls (p&lt;0.01). MNC adherence to endothelial cells was not blocked by antibodies against the intercellular adhesion molecule-1 (ICAM-1), but was blocked by lymphocyte function-associated antigen-1 (LFA-1). 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source MEDLINE; Taylor & Francis Journals Complete
subjects Adolescent
Adult
Antibodies, Monoclonal - immunology
Biological and medical sciences
Brain - cytology
Cell Adhesion - drug effects
Cell Adhesion Molecules - physiology
Cytokines - pharmacology
Endothelium, Vascular - cytology
Endothelium, Vascular - physiology
Female
Humans
Lymphocytes - physiology
Male
Medical sciences
Middle Aged
Multiple Sclerosis - immunology
Multiple sclerosis and variants. Guillain barré syndrome and other inflammatory polyneuropathies. Leukoencephalitis
Neurology
title Adhesion of Cerebral Endothelial Cells to Lymphocytes from Patients with Multiple Sclerosis
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