Determination of Dextromethorphan in Biological Fluids by Liquid Chromatography by Using Semi-microbore Columns

A sensitive, specific procedure for the determirjation of dextromethorphan in plasma and urine in dogs is described. The use of a semi-microbore column, 2.1 mm i.d. × 25 cm resulted in a detection limit of 1 ng/mL in plasma and 25 ng/mL in urine. PJasma (3 mL) is extracted in CCl4 and the drug is ba...

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Veröffentlicht in:	Journal of pharmaceutical sciences 1984-12, Vol.73 (12), p.1821-1822
Hauptverfasser:	Achari, Raja G., Ederma, Heinz M., Chin, David, Oles, Stanley R.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Biological and medical sciences Body Fluids - analysis Chromatography, High Pressure Liquid Dextromethorphan - analysis Dextromethorphan-HPLC determination Dextromethorphan—HPLC determination, semi-microbore column, dog plasma, urine dog plasma General pharmacology Humans Levorphanol - analogs & derivatives Medical sciences Pharmacology. Drug treatments semi-microbore column Spectrophotometry, Ultraviolet urine
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Zusammenfassung:	A sensitive, specific procedure for the determirjation of dextromethorphan in plasma and urine in dogs is described. The use of a semi-microbore column, 2.1 mm i.d. × 25 cm resulted in a detection limit of 1 ng/mL in plasma and 25 ng/mL in urine. PJasma (3 mL) is extracted in CCl4 and the drug is back-extracted with 3% acetic acid. The chromatographic separation is carried out using a 5-μm particle size octadecyl-bonded column, and the drug is monitored by a fluorescence detector. The results after 10-mg oral administrations of dextromethorphan to dogs are provided.
ISSN:	0022-3549 1520-6017
DOI:	10.1002/jps.2600731241