Locating the Catalytic Water Molecule in Serine Proteases

The water molecule Wat in not likely to act as the catalytic nucleophile in the serine protease mechanism because of the distance involved and because the substrate fills only the primary site of the enzyme.

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Bibliographische Detailangaben
Veröffentlicht in:	Science (American Association for the Advancement of Science) 1993-07, Vol.261 (5121), p.620-622
Hauptverfasser:	Perona, John J., Craik, Charles S., Fletterick, Robert J., Singer, P. T., Smalås, A., Carty, R. P., Mangel, W. F., Sweet, R. M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Active sites Atoms Biochemistry Biophysics Carbon Catalysis Catalysts Chemistry Crystal structure Crystallization Enzymes Hydrogen Bonding Hydrogen bonds Hydrolysis Inhalants Molecules Organic Chemistry Oxygen Personality Protein Conformation Proteolysis Protons Serine Endopeptidases - chemistry Technical Comments Trypsin - chemistry Water Water - analysis X-Ray Diffraction
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Beschreibung
Zusammenfassung:	The water molecule Wat in not likely to act as the catalytic nucleophile in the serine protease mechanism because of the distance involved and because the substrate fills only the primary site of the enzyme.
ISSN:	0036-8075 1095-9203
DOI:	10.1126/science.8342029