A 10 microliter indirect ultramicro ELISA for detection of monoclonal antibodies against human alphafetoprotein
An ultramicro ELISA, which uses 10 microliter of reaction volume developed for quantitation of human alphafetoprotein (AFP), has been adapted to use in screening hybridoma products in order to take advantage of the efficiency and sensitivity of this system. Positive samples were detected with a low...
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Veröffentlicht in: | Hybridoma 1984, Vol.3 (4), p.391-396 |
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creator | Otero, A J Sarracent, J Fernańdez Yero, J L Rodríguez, I |
description | An ultramicro ELISA, which uses 10 microliter of reaction volume developed for quantitation of human alphafetoprotein (AFP), has been adapted to use in screening hybridoma products in order to take advantage of the efficiency and sensitivity of this system. Positive samples were detected with a low background level. |
doi_str_mv | 10.1089/hyb.1984.3.391 |
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subjects | alpha -fetoprotein alpha-Fetoproteins - immunology Animals Antibodies, Monoclonal - analysis Chromatography, Affinity enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Humans hybridoma Hybridomas - immunology Immunoenzyme Techniques Mice Microchemistry monoclonal antibodies |
title | A 10 microliter indirect ultramicro ELISA for detection of monoclonal antibodies against human alphafetoprotein |
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