A 10 microliter indirect ultramicro ELISA for detection of monoclonal antibodies against human alphafetoprotein

An ultramicro ELISA, which uses 10 microliter of reaction volume developed for quantitation of human alphafetoprotein (AFP), has been adapted to use in screening hybridoma products in order to take advantage of the efficiency and sensitivity of this system. Positive samples were detected with a low...

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Veröffentlicht in:Hybridoma 1984, Vol.3 (4), p.391-396
Hauptverfasser: Otero, A J, Sarracent, J, Fernańdez Yero, J L, Rodríguez, I
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container_end_page 396
container_issue 4
container_start_page 391
container_title Hybridoma
container_volume 3
creator Otero, A J
Sarracent, J
Fernańdez Yero, J L
Rodríguez, I
description An ultramicro ELISA, which uses 10 microliter of reaction volume developed for quantitation of human alphafetoprotein (AFP), has been adapted to use in screening hybridoma products in order to take advantage of the efficiency and sensitivity of this system. Positive samples were detected with a low background level.
doi_str_mv 10.1089/hyb.1984.3.391
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subjects alpha -fetoprotein
alpha-Fetoproteins - immunology
Animals
Antibodies, Monoclonal - analysis
Chromatography, Affinity
enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Humans
hybridoma
Hybridomas - immunology
Immunoenzyme Techniques
Mice
Microchemistry
monoclonal antibodies
title A 10 microliter indirect ultramicro ELISA for detection of monoclonal antibodies against human alphafetoprotein
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