A 10 microliter indirect ultramicro ELISA for detection of monoclonal antibodies against human alphafetoprotein

An ultramicro ELISA, which uses 10 microliter of reaction volume developed for quantitation of human alphafetoprotein (AFP), has been adapted to use in screening hybridoma products in order to take advantage of the efficiency and sensitivity of this system. Positive samples were detected with a low...

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Veröffentlicht in:	Hybridoma 1984, Vol.3 (4), p.391-396
Hauptverfasser:	Otero, A J, Sarracent, J, Fernańdez Yero, J L, Rodríguez, I
Format:	Artikel
Sprache:	eng
Schlagworte:	alpha -fetoprotein alpha-Fetoproteins - immunology Animals Antibodies, Monoclonal - analysis Chromatography, Affinity enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Humans hybridoma Hybridomas - immunology Immunoenzyme Techniques Mice Microchemistry monoclonal antibodies
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Beschreibung
Zusammenfassung:	An ultramicro ELISA, which uses 10 microliter of reaction volume developed for quantitation of human alphafetoprotein (AFP), has been adapted to use in screening hybridoma products in order to take advantage of the efficiency and sensitivity of this system. Positive samples were detected with a low background level.
ISSN:	0272-457X 0272-457X
DOI:	10.1089/hyb.1984.3.391