Nucleotide sequence of the 3' terminal region of the LEU2 gene from Saccharomyces cerevisiae

The 3' terminal region from the LEU2 gene of yeast has been sequenced. Two open reading frames (ORF) have been identified, one of which constitutes the 3 ' terminus of β-isopropylmalate dehydrogenase, the product of the LEU2 gene. A noncoding spacer region located between the ORFs contains two consensus-type transcriptional terminators. The terminator-like sequences are oriented in opposing directions on opposite DNA strands. The noncoding spacer region may represent a single terminator for the LEU2 gene or two separate terminators involved in blocking convergent transcription from an as yet unidentified yeast gene.