Role of the TK+ phenotype in the stability of pigeonpox virus recombinant

Insertion of foreign DNA containing the E. coli gpt marker by homologous recombination in the pigeonpox virus (PPV) thymidine kinase (TK) gene and selection for the presence of this DNA in the viral genome produced unstable recombinants after 3 plaque purifications. We highlight the persistence of d...

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Veröffentlicht in:	Archives of virology 1993-01, Vol.131 (3-4), p.431-439
1. Verfasser:	LETELLIER, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacterial Proteins - genetics Base Sequence Biological and medical sciences Cells, Cultured Chickens Cloning, Molecular Crossing Over, Genetic DNA, Viral - chemistry Escherichia coli Proteins Fowlpox virus - enzymology Fowlpox virus - genetics Fowlpox virus - growth & development Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral Genetics Microbiology Molecular Sequence Data Pentosyltransferases Phenotype Plasmids Proteins Recombination, Genetic Thymidine Kinase - genetics Transfection Virology Virus Replication - genetics
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Zusammenfassung:	Insertion of foreign DNA containing the E. coli gpt marker by homologous recombination in the pigeonpox virus (PPV) thymidine kinase (TK) gene and selection for the presence of this DNA in the viral genome produced unstable recombinants after 3 plaque purifications. We highlight the persistence of duplicated TK DNA sequences arising from single crossing over, due to the growth advantage of TK+ virus. Restoration of the TK function by coinsertion of the vaccinia virus TK gene led to stable TK+ recombinants arising from double crossing over.
ISSN:	0304-8608 1432-8798
DOI:	10.1007/BF01378643