A Liquid Chromatographic Procedure for the Analysis of Yohimbine in Equine Serum and Urine

A standardbred mare was dosed with 40 mg yohimbine intravenously. Serum and urine samples were collected and analyzed for yohimbine using solvent extraction and reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection. Maximum yohimbine concentrations of 45 and 18 ng/...

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Veröffentlicht in:	Journal of analytical toxicology 1993-05, Vol.17 (3), p.178-181
Hauptverfasser:	Reimer, Gerry, Suarez, Agripina, Chui, Y.C.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Animals Biological and medical sciences Chromatography, High Pressure Liquid Chromatography, Liquid Female Fluorescence General pharmacology Half-Life High-performance liquid chromatography Horses Liquid chromatography Mass Spectrometry Medical sciences Pharmacology. Drug treatments Solvents Spectrometry, Fluorescence Urine yohimbine Yohimbine - analysis Yohimbine - blood Yohimbine - urine
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Beschreibung
Zusammenfassung:	A standardbred mare was dosed with 40 mg yohimbine intravenously. Serum and urine samples were collected and analyzed for yohimbine using solvent extraction and reversed-phase high-performance liquid chromatography (HPLC) with fluorescence detection. Maximum yohimbine concentrations of 45 and 18 ng/mL were observed in serum and urine samples, respectively. Elimination was rapid, with half-lives of approximately 20 and 53 min observed for serum and urine, respectively. The presence of yohimbine in these samples was confirmed by liquid chromatography/mass spectroscopy (LC/MS/MS).
ISSN:	0146-4760 1945-2403
DOI:	10.1093/jat/17.3.178