Mode of degradation of plasmid DNA with ozone

The ozonization of pBR322 closed circular DNA showed the conversion to open circular DNA. The damaged site was investigated by restriction mapping. The results showed the damage and subsequent cleavage of the DNA strand of ccDNA by ozonization may occur at the region sensitive to nuclease S1.

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Bibliographische Detailangaben
Veröffentlicht in:	Nucleic acids symposium series (1979) 1984 (15), p.151-154
Hauptverfasser:	Sawadaishi, K, Miura, K, Ohthuka, E, Ueda, T, Ishizaki, K, Shinriki, N
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle DNA DNA Restriction Enzymes DNA, Bacterial Electrophoresis, Agar Gel - methods Escherichia coli - genetics Ozone Plasmids Thymus Gland
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Beschreibung
Zusammenfassung:	The ozonization of pBR322 closed circular DNA showed the conversion to open circular DNA. The damaged site was investigated by restriction mapping. The results showed the damage and subsequent cleavage of the DNA strand of ccDNA by ozonization may occur at the region sensitive to nuclease S1.
ISSN:	0261-3166