The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and Ecohi

The cleavage sites and localization of genes encoding the restriction endonucleases Eco1831I and Ecohi

The restriction endonucleases Eco 1831I and Ecohi cleave before the first 5'-cytosine in the recognition sequence 5' -↓ CCSGG --3' 3' --GGSCC↑-5'(where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA + ho...

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Veröffentlicht in:Gene 1993-07, Vol.129 (1), p.153-154
Hauptverfasser: Kravetz, Anatoly N., Zakharova, Marina V., Beletskaya, Irina V., Sineva, Elena V., Denjmuchametov, Marat M., Petrov, Sergey I., Glatman, Larisa I., Solonin, Alexander S.
Format: Artikel
Sprache:eng
Schlagworte:
Cloning, Molecular
Deoxyribonucleases, Type II Site-Specific - genetics
Deoxyribonucleases, Type II Site-Specific - metabolism
enzyme identification
Escherichia coli
Escherichia coli - enzymology
Genes, Bacterial
McrA
nucleotide sequence
plasmids
Substrate Specificity
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Zusammenfassung:The restriction endonucleases Eco 1831I and Ecohi cleave before the first 5'-cytosine in the recognition sequence 5' -↓ CCSGG --3' 3' --GGSCC↑-5'(where S = G or C), generate 5-base 5' cohesive ends, and are encoded by homologous plasmids that are restricted in McrA + hosts. Thus, they differ in their cleavage specificity from that of the BcnI isoschizomer, which cleaves after the second 5' cytosine.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(93)90712-C