Ion-Pair High-Performance Liquid Chromatographic Analysis of Sulpyrine and Its Metabolites in Rabbit Plasma

A simple, specific and sensitive ion-pair high-performance liquid chromatographic analysis was developed for aminopyrine, sulpyrine and their metabolites (4-methylaminoantipyrine, 4-aminoantipyrine, 4-acetylaminoantipyrine and 4-formylaminoantipyrine) in rabbit plasma. Following deproteinization wit...

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Veröffentlicht in:Chemical & pharmaceutical bulletin 1984/08/25, Vol.32(8), pp.3194-3198
Hauptverfasser: ITOH, SOICHI, TANABE, KAZUHISA, FURUICHI, YUMI, SUZUKA, TAKUMI, KUBO, KAZUYOSHI, YAMAZAKI, MASARU, KAMADA, AKIRA
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Sprache:eng
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Zusammenfassung:A simple, specific and sensitive ion-pair high-performance liquid chromatographic analysis was developed for aminopyrine, sulpyrine and their metabolites (4-methylaminoantipyrine, 4-aminoantipyrine, 4-acetylaminoantipyrine and 4-formylaminoantipyrine) in rabbit plasma. Following deproteinization with acetonitrile and separation on an octadecylsilane-bonded silica column (250×4mm, JASCO Fine SIL C-18), the eluted components were detected with a multiwavelength ultraviolet monitor at 260nm. The mobile phase consisted of 22% (v/v) acetonitrile and 78% (v/v) of an aqueous solution containing 10mM KH2PO4 and 1.24mM tetra-n-butylammonium bromide, adjusted to pH 4.5 with 0.1M phosphoric acid solution. Hexobarbital was employed as an internal standard. Based on 0.1ml of plasma, the detection limits were 0.35μM for 4-acetylaminoantipyrine, 0.35μM for 4-formylaminoantipyrine, 0.7μM for sulpyrine, 1μM for 4-aminoantipyrine, and 2μM for 4-methylaminoantipyrine at the signal-to-noise ratio of 5 : 1.
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.32.3194