Crystallization and Preliminary Crystallographic Study of Citrate Synthase from the Thermophilic Archaeon Thermoplasma acidophilum
Single crystals of citrate synthase from the Archaeon Thermoplasma acidophilum were obtained in two forms rasing the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group P222 1, with unit cell dimensions a = 80·9 Å,...
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| Veröffentlicht in: | Journal of molecular biology 1993-07, Vol.232 (1), p.308-309 |
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| container_title | Journal of molecular biology |
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| creator | Russell, Rupert J.M. Byrom, David Danson, Michael J. Hough, David W. Taylor, Garry L. |
| description | Single crystals of citrate synthase from the
Archaeon Thermoplasma acidophilum were obtained in two forms rasing the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group
P222
1, with unit cell dimensions
a = 80·9 Å,
b = 103·8 Å,
c = 98·3 Å and one dimer in the asymmetric unit. Type 2 crystals belong to the monoclinic space group
P2
1, with unit cell dimensions
a = 53·8 Å,
b = 173·8 Å,
c = 86·7 Å and β = 97·1° and two dimers in the asymmetric unit. |
| doi_str_mv | 10.1006/jmbi.1993.1386 |
| format | Article |
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Archaeon Thermoplasma acidophilum were obtained in two forms rasing the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group
P222
1, with unit cell dimensions
a = 80·9 Å,
b = 103·8 Å,
c = 98·3 Å and one dimer in the asymmetric unit. Type 2 crystals belong to the monoclinic space group
P2
1, with unit cell dimensions
a = 53·8 Å,
b = 173·8 Å,
c = 86·7 Å and β = 97·1° and two dimers in the asymmetric unit.</description><identifier>ISSN: 0022-2836</identifier><identifier>EISSN: 1089-8638</identifier><identifier>DOI: 10.1006/jmbi.1993.1386</identifier><identifier>PMID: 8331668</identifier><identifier>CODEN: JMOBAK</identifier><language>eng</language><publisher>Oxford: Elsevier Ltd</publisher><subject>Analytical, structural and metabolic biochemistry ; archaea ; Biological and medical sciences ; Citrate (si)-Synthase - isolation & purification ; Citrate (si)-Synthase - ultrastructure ; citrate synthase ; Crystallography ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Lyases ; Protein Conformation ; Thermoplasma - enzymology ; Thermoplasma acidophilum ; X-Ray Diffraction</subject><ispartof>Journal of molecular biology, 1993-07, Vol.232 (1), p.308-309</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c399t-dc175eb99dbafb668dcc2040355c29d67369389ecbadbc8e46acc74d3e0edb5f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/jmbi.1993.1386$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4827849$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8331668$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Russell, Rupert J.M.</creatorcontrib><creatorcontrib>Byrom, David</creatorcontrib><creatorcontrib>Danson, Michael J.</creatorcontrib><creatorcontrib>Hough, David W.</creatorcontrib><creatorcontrib>Taylor, Garry L.</creatorcontrib><title>Crystallization and Preliminary Crystallographic Study of Citrate Synthase from the Thermophilic Archaeon Thermoplasma acidophilum</title><title>Journal of molecular biology</title><addtitle>J Mol Biol</addtitle><description>Single crystals of citrate synthase from the
Archaeon Thermoplasma acidophilum were obtained in two forms rasing the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group
P222
1, with unit cell dimensions
a = 80·9 Å,
b = 103·8 Å,
c = 98·3 Å and one dimer in the asymmetric unit. Type 2 crystals belong to the monoclinic space group
P2
1, with unit cell dimensions
a = 53·8 Å,
b = 173·8 Å,
c = 86·7 Å and β = 97·1° and two dimers in the asymmetric unit.</description><subject>Analytical, structural and metabolic biochemistry</subject><subject>archaea</subject><subject>Biological and medical sciences</subject><subject>Citrate (si)-Synthase - isolation & purification</subject><subject>Citrate (si)-Synthase - ultrastructure</subject><subject>citrate synthase</subject><subject>Crystallography</subject><subject>Enzymes and enzyme inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lyases</subject><subject>Protein Conformation</subject><subject>Thermoplasma - enzymology</subject><subject>Thermoplasma acidophilum</subject><subject>X-Ray Diffraction</subject><issn>0022-2836</issn><issn>1089-8638</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEUx4NY6lq9ehNyEG-zJpOZTHIsi61CQaHtObxJ3jgp82NNMsL26F9utjv2Jp4C-X7el8f7EPKOsy1nTH56GFu_5VqLLRdKviAbzpQulBTqJdkwVpZFqYR8RV7H-MAYq0Wlzsm5EoJLqTbk9y4cYoJh8I-Q_DxRmBz9HnDwo58gHOjffP4RYN97S2_T4g507ujOpwAJ6e1hSj1EpF2YR5p6pHc9hnHO9JD5y2B7wNy8_g4QR6BgvXsilvENOetgiPh2fS_I_dXnu92X4ubb9dfd5U1hhdapcJY3NbZauxa6Ni_vrC1ZxURd21I72QiphdJoW3CtVVhJsLapnECGrq07cUE-nnr3Yf65YExm9NHiMMCE8xJNUyuhmaz_C3KpuBS6yeD2BNowxxiwM_vgx3w1w5k52jFHO-Zoxxzt5IH3a_PSjuie8VVHzj-sOUQLQxdgsj4-Y5UqG1XpjKkThvlcvzwGE63HyaLzAW0ybvb_2uAPeXSvrw</recordid><startdate>19930705</startdate><enddate>19930705</enddate><creator>Russell, Rupert J.M.</creator><creator>Byrom, David</creator><creator>Danson, Michael J.</creator><creator>Hough, David W.</creator><creator>Taylor, Garry L.</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19930705</creationdate><title>Crystallization and Preliminary Crystallographic Study of Citrate Synthase from the Thermophilic Archaeon Thermoplasma acidophilum</title><author>Russell, Rupert J.M. ; Byrom, David ; Danson, Michael J. ; Hough, David W. ; Taylor, Garry L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c399t-dc175eb99dbafb668dcc2040355c29d67369389ecbadbc8e46acc74d3e0edb5f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Analytical, structural and metabolic biochemistry</topic><topic>archaea</topic><topic>Biological and medical sciences</topic><topic>Citrate (si)-Synthase - isolation & purification</topic><topic>Citrate (si)-Synthase - ultrastructure</topic><topic>citrate synthase</topic><topic>Crystallography</topic><topic>Enzymes and enzyme inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lyases</topic><topic>Protein Conformation</topic><topic>Thermoplasma - enzymology</topic><topic>Thermoplasma acidophilum</topic><topic>X-Ray Diffraction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Russell, Rupert J.M.</creatorcontrib><creatorcontrib>Byrom, David</creatorcontrib><creatorcontrib>Danson, Michael J.</creatorcontrib><creatorcontrib>Hough, David W.</creatorcontrib><creatorcontrib>Taylor, Garry L.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Russell, Rupert J.M.</au><au>Byrom, David</au><au>Danson, Michael J.</au><au>Hough, David W.</au><au>Taylor, Garry L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Crystallization and Preliminary Crystallographic Study of Citrate Synthase from the Thermophilic Archaeon Thermoplasma acidophilum</atitle><jtitle>Journal of molecular biology</jtitle><addtitle>J Mol Biol</addtitle><date>1993-07-05</date><risdate>1993</risdate><volume>232</volume><issue>1</issue><spage>308</spage><epage>309</epage><pages>308-309</pages><issn>0022-2836</issn><eissn>1089-8638</eissn><coden>JMOBAK</coden><abstract>Single crystals of citrate synthase from the
Archaeon Thermoplasma acidophilum were obtained in two forms rasing the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group
P222
1, with unit cell dimensions
a = 80·9 Å,
b = 103·8 Å,
c = 98·3 Å and one dimer in the asymmetric unit. Type 2 crystals belong to the monoclinic space group
P2
1, with unit cell dimensions
a = 53·8 Å,
b = 173·8 Å,
c = 86·7 Å and β = 97·1° and two dimers in the asymmetric unit.</abstract><cop>Oxford</cop><pub>Elsevier Ltd</pub><pmid>8331668</pmid><doi>10.1006/jmbi.1993.1386</doi><tpages>2</tpages></addata></record> |
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| ispartof | Journal of molecular biology, 1993-07, Vol.232 (1), p.308-309 |
| issn | 0022-2836 1089-8638 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Analytical, structural and metabolic biochemistry archaea Biological and medical sciences Citrate (si)-Synthase - isolation & purification Citrate (si)-Synthase - ultrastructure citrate synthase Crystallography Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Lyases Protein Conformation Thermoplasma - enzymology Thermoplasma acidophilum X-Ray Diffraction |
| title | Crystallization and Preliminary Crystallographic Study of Citrate Synthase from the Thermophilic Archaeon Thermoplasma acidophilum |
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