Crystallization and Preliminary Crystallographic Study of Citrate Synthase from the Thermophilic Archaeon Thermoplasma acidophilum

Single crystals of citrate synthase from the Archaeon Thermoplasma acidophilum were obtained in two forms rasing the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group P222 1, with unit cell dimensions a = 80·9 Å,...

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Veröffentlicht in:	Journal of molecular biology 1993-07, Vol.232 (1), p.308-309
Hauptverfasser:	Russell, Rupert J.M., Byrom, David, Danson, Michael J., Hough, David W., Taylor, Garry L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry archaea Biological and medical sciences Citrate (si)-Synthase - isolation & purification Citrate (si)-Synthase - ultrastructure citrate synthase Crystallography Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Lyases Protein Conformation Thermoplasma - enzymology Thermoplasma acidophilum X-Ray Diffraction
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Beschreibung
Zusammenfassung:	Single crystals of citrate synthase from the Archaeon Thermoplasma acidophilum were obtained in two forms rasing the hanging drop vapour diffusion method and polyethylene glycol 3350 as precipitant. Type 1 crystals belong to the orthorhombic space group P222 1, with unit cell dimensions a = 80·9 Å, b = 103·8 Å, c = 98·3 Å and one dimer in the asymmetric unit. Type 2 crystals belong to the monoclinic space group P2 1, with unit cell dimensions a = 53·8 Å, b = 173·8 Å, c = 86·7 Å and β = 97·1° and two dimers in the asymmetric unit.
ISSN:	0022-2836 1089-8638
DOI:	10.1006/jmbi.1993.1386