NK-1 tachykinin receptor in rat and guinea pig brains: Pharmacological and autoradiographical evidence for a species difference

The affinities of a large variety of peptide or nonpeptide tachykinin analogues were determined on membranes from rat and guinea pig brains using the selective NK-1 radioligand 3H-[Pro 9]SP. Nonpeptide antagonists clearly revealed a species difference; (±)CP-96,345 was more potent in the guinea pig,...

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Veröffentlicht in:	Peptides (New York, N.Y. : 1980) N.Y. : 1980), 1993-05, Vol.14 (3), p.551-559
Hauptverfasser:	Petitet, François, Beaujouan, Jean-Claude, Saffroy, Monique, Torrens, Yvette, Fardin, Véronique, Glowinski, Jacques
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Autoradiography Binding studies Binding, Competitive - physiology Brain Chemistry - drug effects Guinea Pigs In Vitro Techniques Male NK-1 receptor Radioligand Assay Rats Rats, Sprague-Dawley Receptors, Neurokinin-2 Receptors, Neurotransmitter - analysis Receptors, Neurotransmitter - drug effects Receptors, Neurotransmitter - metabolism Species difference Species Specificity Tachykinins Tachykinins - metabolism
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Zusammenfassung:	The affinities of a large variety of peptide or nonpeptide tachykinin analogues were determined on membranes from rat and guinea pig brains using the selective NK-1 radioligand 3H-[Pro 9]SP. Nonpeptide antagonists clearly revealed a species difference; (±)CP-96,345 was more potent in the guinea pig, while RP 67580 was found to be a better competitor of 3H-[Pro 9]SP binding to rat brain membranes. This was confirmed on brain slices by autoradiography. Numerous brain structures were analyzed by optical densitometry. From these data, a heterogeneity of NK-1 binding sites among different structures can be excluded in both rat and guinea pig brains.
ISSN:	0196-9781 1873-5169
DOI:	10.1016/0196-9781(93)90144-6