An improved method for preparing DNA from human brain
The preparation of DNA from human brain by conventional protease and RNAase digestion, phenol-chloroform extraction and ethanol precipitation results in very low yields. This is probably due to interference by lipid present at very high levels in this tissue. We have overcome this by solubilizing th...
Gespeichert in:
| Veröffentlicht in: | Journal of neuroscience methods 1984-01, Vol.11 (4), p.275-279 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 279 |
|---|---|
| container_issue | 4 |
| container_start_page | 275 |
| container_title | Journal of neuroscience methods |
| container_volume | 11 |
| creator | Saldanha, John Gannicliffe, Alison Itzhaki, Ruth F. |
| description | The preparation of DNA from human brain by conventional protease and RNAase digestion, phenol-chloroform extraction and ethanol precipitation results in very low yields. This is probably due to interference by lipid present at very high levels in this tissue. We have overcome this by solubilizing the initial tissue homogenate by heating at 60°C for 30 min in sodium dodecyl sulphate. This results in a threefold increase in yield and a considerable reduction in preparation time. The molecular weight of the DNA is greater than 20 × 10
6 milion. |
| doi_str_mv | 10.1016/0165-0270(84)90089-X |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75826114</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>016502708490089X</els_id><sourcerecordid>14054612</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-fa7693f199d3fac2767217baac7233f68d3e72c9635e50e1293c2da438105b533</originalsourceid><addsrcrecordid>eNqFkE1LAzEQhoMotVb_gcIeRPSwmq_Nx0Uo9ROKXhR6C2l2YiPd3Zp0C_57t7b0qIdhDvO8w8yD0CnB1wQTcdNVkWMq8aXiVxpjpfPJHuoTJWkupJrso_4OOURHKX1ijLnGood6AmshmOqjYlhnoVrEZgVlVsFy1pSZb2K2iLCwMdQf2d3LMPOxqbJZW9k6m0Yb6mN04O08wcm2D9D7w_3b6Ckfvz4-j4bj3HEil7m3UmjmidYl89ZRKSQlcmqtk5QxL1TJQFKnBSugwECoZo6WljNFcDEtGBugi83e7sCvFtLSVCE5mM9tDU2bjCwUFYTwf0HCccEFoR3IN6CLTUoRvFnEUNn4bQg2a61m7cysnRnFza9WM-liZ9v97bSCchfaeuzm59u5Tc7OfbS1C2mHKaW6h3GH3W4w6KStAkSTXIDaQRkiuKUpm_D3HT-zTZEn</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14054612</pqid></control><display><type>article</type><title>An improved method for preparing DNA from human brain</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Saldanha, John ; Gannicliffe, Alison ; Itzhaki, Ruth F.</creator><creatorcontrib>Saldanha, John ; Gannicliffe, Alison ; Itzhaki, Ruth F.</creatorcontrib><description>The preparation of DNA from human brain by conventional protease and RNAase digestion, phenol-chloroform extraction and ethanol precipitation results in very low yields. This is probably due to interference by lipid present at very high levels in this tissue. We have overcome this by solubilizing the initial tissue homogenate by heating at 60°C for 30 min in sodium dodecyl sulphate. This results in a threefold increase in yield and a considerable reduction in preparation time. The molecular weight of the DNA is greater than 20 × 10
6 milion.</description><identifier>ISSN: 0165-0270</identifier><identifier>EISSN: 1872-678X</identifier><identifier>DOI: 10.1016/0165-0270(84)90089-X</identifier><identifier>PMID: 6096638</identifier><identifier>CODEN: JNMEDT</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Biochemistry and metabolism ; Biological and medical sciences ; Brain Chemistry ; Central nervous system ; DNA - analysis ; DNA - metabolism ; DNA preparation ; Fundamental and applied biological sciences. Psychology ; human brain ; Humans ; Mental Disorders - metabolism ; Methods ; Simplexvirus - genetics ; sodium dodecyl sulphate ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neuroscience methods, 1984-01, Vol.11 (4), p.275-279</ispartof><rights>1984</rights><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-fa7693f199d3fac2767217baac7233f68d3e72c9635e50e1293c2da438105b533</citedby><cites>FETCH-LOGICAL-c417t-fa7693f199d3fac2767217baac7233f68d3e72c9635e50e1293c2da438105b533</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0165-0270(84)90089-X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8882330$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6096638$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Saldanha, John</creatorcontrib><creatorcontrib>Gannicliffe, Alison</creatorcontrib><creatorcontrib>Itzhaki, Ruth F.</creatorcontrib><title>An improved method for preparing DNA from human brain</title><title>Journal of neuroscience methods</title><addtitle>J Neurosci Methods</addtitle><description>The preparation of DNA from human brain by conventional protease and RNAase digestion, phenol-chloroform extraction and ethanol precipitation results in very low yields. This is probably due to interference by lipid present at very high levels in this tissue. We have overcome this by solubilizing the initial tissue homogenate by heating at 60°C for 30 min in sodium dodecyl sulphate. This results in a threefold increase in yield and a considerable reduction in preparation time. The molecular weight of the DNA is greater than 20 × 10
6 milion.</description><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Brain Chemistry</subject><subject>Central nervous system</subject><subject>DNA - analysis</subject><subject>DNA - metabolism</subject><subject>DNA preparation</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>human brain</subject><subject>Humans</subject><subject>Mental Disorders - metabolism</subject><subject>Methods</subject><subject>Simplexvirus - genetics</subject><subject>sodium dodecyl sulphate</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0165-0270</issn><issn>1872-678X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1984</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1LAzEQhoMotVb_gcIeRPSwmq_Nx0Uo9ROKXhR6C2l2YiPd3Zp0C_57t7b0qIdhDvO8w8yD0CnB1wQTcdNVkWMq8aXiVxpjpfPJHuoTJWkupJrso_4OOURHKX1ijLnGood6AmshmOqjYlhnoVrEZgVlVsFy1pSZb2K2iLCwMdQf2d3LMPOxqbJZW9k6m0Yb6mN04O08wcm2D9D7w_3b6Ckfvz4-j4bj3HEil7m3UmjmidYl89ZRKSQlcmqtk5QxL1TJQFKnBSugwECoZo6WljNFcDEtGBugi83e7sCvFtLSVCE5mM9tDU2bjCwUFYTwf0HCccEFoR3IN6CLTUoRvFnEUNn4bQg2a61m7cysnRnFza9WM-liZ9v97bSCchfaeuzm59u5Tc7OfbS1C2mHKaW6h3GH3W4w6KStAkSTXIDaQRkiuKUpm_D3HT-zTZEn</recordid><startdate>19840101</startdate><enddate>19840101</enddate><creator>Saldanha, John</creator><creator>Gannicliffe, Alison</creator><creator>Itzhaki, Ruth F.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19840101</creationdate><title>An improved method for preparing DNA from human brain</title><author>Saldanha, John ; Gannicliffe, Alison ; Itzhaki, Ruth F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-fa7693f199d3fac2767217baac7233f68d3e72c9635e50e1293c2da438105b533</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1984</creationdate><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Brain Chemistry</topic><topic>Central nervous system</topic><topic>DNA - analysis</topic><topic>DNA - metabolism</topic><topic>DNA preparation</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>human brain</topic><topic>Humans</topic><topic>Mental Disorders - metabolism</topic><topic>Methods</topic><topic>Simplexvirus - genetics</topic><topic>sodium dodecyl sulphate</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saldanha, John</creatorcontrib><creatorcontrib>Gannicliffe, Alison</creatorcontrib><creatorcontrib>Itzhaki, Ruth F.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neuroscience methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saldanha, John</au><au>Gannicliffe, Alison</au><au>Itzhaki, Ruth F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An improved method for preparing DNA from human brain</atitle><jtitle>Journal of neuroscience methods</jtitle><addtitle>J Neurosci Methods</addtitle><date>1984-01-01</date><risdate>1984</risdate><volume>11</volume><issue>4</issue><spage>275</spage><epage>279</epage><pages>275-279</pages><issn>0165-0270</issn><eissn>1872-678X</eissn><coden>JNMEDT</coden><abstract>The preparation of DNA from human brain by conventional protease and RNAase digestion, phenol-chloroform extraction and ethanol precipitation results in very low yields. This is probably due to interference by lipid present at very high levels in this tissue. We have overcome this by solubilizing the initial tissue homogenate by heating at 60°C for 30 min in sodium dodecyl sulphate. This results in a threefold increase in yield and a considerable reduction in preparation time. The molecular weight of the DNA is greater than 20 × 10
6 milion.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>6096638</pmid><doi>10.1016/0165-0270(84)90089-X</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0165-0270 |
| ispartof | Journal of neuroscience methods, 1984-01, Vol.11 (4), p.275-279 |
| issn | 0165-0270 1872-678X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75826114 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Biochemistry and metabolism Biological and medical sciences Brain Chemistry Central nervous system DNA - analysis DNA - metabolism DNA preparation Fundamental and applied biological sciences. Psychology human brain Humans Mental Disorders - metabolism Methods Simplexvirus - genetics sodium dodecyl sulphate Vertebrates: nervous system and sense organs |
| title | An improved method for preparing DNA from human brain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-23T02%3A45%3A45IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20improved%20method%20for%20preparing%20DNA%20from%20human%20brain&rft.jtitle=Journal%20of%20neuroscience%20methods&rft.au=Saldanha,%20John&rft.date=1984-01-01&rft.volume=11&rft.issue=4&rft.spage=275&rft.epage=279&rft.pages=275-279&rft.issn=0165-0270&rft.eissn=1872-678X&rft.coden=JNMEDT&rft_id=info:doi/10.1016/0165-0270(84)90089-X&rft_dat=%3Cproquest_cross%3E14054612%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14054612&rft_id=info:pmid/6096638&rft_els_id=016502708490089X&rfr_iscdi=true |