An improved method for preparing DNA from human brain

The preparation of DNA from human brain by conventional protease and RNAase digestion, phenol-chloroform extraction and ethanol precipitation results in very low yields. This is probably due to interference by lipid present at very high levels in this tissue. We have overcome this by solubilizing th...

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Veröffentlicht in:	Journal of neuroscience methods 1984-01, Vol.11 (4), p.275-279
Hauptverfasser:	Saldanha, John, Gannicliffe, Alison, Itzhaki, Ruth F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Biochemistry and metabolism Biological and medical sciences Brain Chemistry Central nervous system DNA - analysis DNA - metabolism DNA preparation Fundamental and applied biological sciences. Psychology human brain Humans Mental Disorders - metabolism Methods Simplexvirus - genetics sodium dodecyl sulphate Vertebrates: nervous system and sense organs
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Zusammenfassung:	The preparation of DNA from human brain by conventional protease and RNAase digestion, phenol-chloroform extraction and ethanol precipitation results in very low yields. This is probably due to interference by lipid present at very high levels in this tissue. We have overcome this by solubilizing the initial tissue homogenate by heating at 60°C for 30 min in sodium dodecyl sulphate. This results in a threefold increase in yield and a considerable reduction in preparation time. The molecular weight of the DNA is greater than 20 × 10 6 milion.
ISSN:	0165-0270 1872-678X
DOI:	10.1016/0165-0270(84)90089-X