Levels of synovial fluid interleukin‐1 receptor antagonist in rheumatoid arthritis and other arthropathies. potential contribution from synovial fluid neutrophils

Objective. To measure synovial fluid (SF) levels of interleukin‐1 receptor antagonist (IL‐1ra) and to determine the capacity of SF neutrophils (PMN) to synthesize and release IL‐1ra. Methods. A sensitive and specific enzyme‐linked immunosorbent assay was used to measure SF IL‐1ra protein concentrati...

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Veröffentlicht in:Arthritis and rheumatism 1993-06, Vol.36 (6), p.781-789
Hauptverfasser: Malyak, Mark, Swaney, Robert E., Arend, William P.
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Sprache:eng
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Zusammenfassung:Objective. To measure synovial fluid (SF) levels of interleukin‐1 receptor antagonist (IL‐1ra) and to determine the capacity of SF neutrophils (PMN) to synthesize and release IL‐1ra. Methods. A sensitive and specific enzyme‐linked immunosorbent assay was used to measure SF IL‐1ra protein concentrations and IL‐1ra production by isolated SF PMN. Results. SF IL‐1ra levels were elevated in 13 of 16 samples from patients with rheumatoid arthritis (RA) (mean 17.1 ng/ml), in 6 of 18 samples from patients with infectious or inflammatory, non‐RA arthropathies (mean 10.6 ng/ml), and in none of 11 noninflammatory SF samples. SF IL‐1ra levels correlated with SF PMN concentrations (r = 0.680, P < 0.00001). Isolated SF PMN contained preexisting IL‐1ra protein in the absence of messenger RNA (mRNA). In addition, both lipopolysaccharide and granulocyte‐macrophage colonystimulating factor induced modest increases in IL‐1ra mRNA by cultured SF PMN. Conclusion. IL‐1ra levels are increased in >80% of RA SF samples. SF PMN produce IL‐1ra, possibly contributing to the levels of IL‐1ra present within the SF.
ISSN:0004-3591
1529-0131
DOI:10.1002/art.1780360607