Development of Laser-Excited Indirect Fluorescence Detection for High-Molecular-Weight Polysaccharides in Capillary Electrophoresis
The separation and detection of native polysaccharides in capillary zone electrophoresis is presented. To provide differences in the electrophoretic mobility, a pH of 11.5 is used to partially ionize the analytes. Detection is made possible by indirect fluorescence, where the charged analytes displa...
Gespeichert in:
| Veröffentlicht in: | Analytical biochemistry 1993-05, Vol.210 (2), p.245-248 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 248 |
|---|---|
| container_issue | 2 |
| container_start_page | 245 |
| container_title | Analytical biochemistry |
| container_volume | 210 |
| creator | Richmond, M.D. Yeung, E.S. |
| description | The separation and detection of native polysaccharides in capillary zone electrophoresis is presented. To provide differences in the electrophoretic mobility, a pH of 11.5 is used to partially ionize the analytes. Detection is made possible by indirect fluorescence, where the charged analytes displace the anionic fluorescing buffer ion to create negative peaks. Detection limits in the picogram range are possible. |
| doi_str_mv | 10.1006/abio.1993.1190 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75777052</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0003269783711905</els_id><sourcerecordid>75777052</sourcerecordid><originalsourceid>FETCH-LOGICAL-c434t-b4855f8b27acc2842926bd48ce30243ccc0abb2f122e43f213fa2eb740bfa5783</originalsourceid><addsrcrecordid>eNp1kUtv3CAURlHUKp08ttlVYlF15wkvG3tZTSYPaaJ20ShLBPjSofIYF-yoWeePB2dG2WWF4B4-fRwQuqBkSQmpLrXxYUmbhi8pbcgRWlDSVAXhpPmEFoQQXrCqkV_QSUp_CaFUlNUxOpZVXZaVWKCXK3iCLgw76EccHN7oBLFY_7d-hBbf9a2PYEd83U0hQrLQW8BXMOYzH3rsQsS3_s-2uA8d2KnTsXiEvB_xr9A9J23tVkffQsK-xys9-C4jz3id4TGGYTtn-nSGPjvdJTg_rKfo4Xr9e3VbbH7e3K1-bAoruBgLI3JnVxsmcy6rBWtYZVpRW-CECW6tJdoY5ihjILhjlDvNwEhBjNOlrPkp-r7PHWL4N0Ea1c7nJ-VOPYQpKVlKKUnJMrjcgzaGlCI4NUS_y80VJWq2rmbrarauZuv5wtdD8mR20L7jB815_u0w18nqzkXdW5_eMSF5zd-weo9BtvDkIapk_ax8_wuqDf6jBq8BR6CG</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>75777052</pqid></control><display><type>article</type><title>Development of Laser-Excited Indirect Fluorescence Detection for High-Molecular-Weight Polysaccharides in Capillary Electrophoresis</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Richmond, M.D. ; Yeung, E.S.</creator><creatorcontrib>Richmond, M.D. ; Yeung, E.S.</creatorcontrib><description>The separation and detection of native polysaccharides in capillary zone electrophoresis is presented. To provide differences in the electrophoretic mobility, a pH of 11.5 is used to partially ionize the analytes. Detection is made possible by indirect fluorescence, where the charged analytes displace the anionic fluorescing buffer ion to create negative peaks. Detection limits in the picogram range are possible.</description><identifier>ISSN: 0003-2697</identifier><identifier>EISSN: 1096-0309</identifier><identifier>DOI: 10.1006/abio.1993.1190</identifier><identifier>PMID: 7685564</identifier><identifier>CODEN: ANBCA2</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Amylose - analogs & derivatives ; Amylose - analysis ; Amylose - isolation & purification ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Carbohydrates ; Dextrans - analysis ; Dextrans - isolation & purification ; Electrophoresis - methods ; Fundamental and applied biological sciences. Psychology ; Holosides ; Hydrogen-Ion Concentration ; Lasers ; Microchemistry - methods ; Microscopy, Fluorescence - methods ; Molecular Weight ; Other biological molecules ; Polysaccharides - analysis ; Polysaccharides - isolation & purification ; Sensitivity and Specificity</subject><ispartof>Analytical biochemistry, 1993-05, Vol.210 (2), p.245-248</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c434t-b4855f8b27acc2842926bd48ce30243ccc0abb2f122e43f213fa2eb740bfa5783</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/abio.1993.1190$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4738364$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7685564$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Richmond, M.D.</creatorcontrib><creatorcontrib>Yeung, E.S.</creatorcontrib><title>Development of Laser-Excited Indirect Fluorescence Detection for High-Molecular-Weight Polysaccharides in Capillary Electrophoresis</title><title>Analytical biochemistry</title><addtitle>Anal Biochem</addtitle><description>The separation and detection of native polysaccharides in capillary zone electrophoresis is presented. To provide differences in the electrophoretic mobility, a pH of 11.5 is used to partially ionize the analytes. Detection is made possible by indirect fluorescence, where the charged analytes displace the anionic fluorescing buffer ion to create negative peaks. Detection limits in the picogram range are possible.</description><subject>Amylose - analogs & derivatives</subject><subject>Amylose - analysis</subject><subject>Amylose - isolation & purification</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Carbohydrates</subject><subject>Dextrans - analysis</subject><subject>Dextrans - isolation & purification</subject><subject>Electrophoresis - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Holosides</subject><subject>Hydrogen-Ion Concentration</subject><subject>Lasers</subject><subject>Microchemistry - methods</subject><subject>Microscopy, Fluorescence - methods</subject><subject>Molecular Weight</subject><subject>Other biological molecules</subject><subject>Polysaccharides - analysis</subject><subject>Polysaccharides - isolation & purification</subject><subject>Sensitivity and Specificity</subject><issn>0003-2697</issn><issn>1096-0309</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUtv3CAURlHUKp08ttlVYlF15wkvG3tZTSYPaaJ20ShLBPjSofIYF-yoWeePB2dG2WWF4B4-fRwQuqBkSQmpLrXxYUmbhi8pbcgRWlDSVAXhpPmEFoQQXrCqkV_QSUp_CaFUlNUxOpZVXZaVWKCXK3iCLgw76EccHN7oBLFY_7d-hBbf9a2PYEd83U0hQrLQW8BXMOYzH3rsQsS3_s-2uA8d2KnTsXiEvB_xr9A9J23tVkffQsK-xys9-C4jz3id4TGGYTtn-nSGPjvdJTg_rKfo4Xr9e3VbbH7e3K1-bAoruBgLI3JnVxsmcy6rBWtYZVpRW-CECW6tJdoY5ihjILhjlDvNwEhBjNOlrPkp-r7PHWL4N0Ea1c7nJ-VOPYQpKVlKKUnJMrjcgzaGlCI4NUS_y80VJWq2rmbrarauZuv5wtdD8mR20L7jB815_u0w18nqzkXdW5_eMSF5zd-weo9BtvDkIapk_ax8_wuqDf6jBq8BR6CG</recordid><startdate>19930501</startdate><enddate>19930501</enddate><creator>Richmond, M.D.</creator><creator>Yeung, E.S.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930501</creationdate><title>Development of Laser-Excited Indirect Fluorescence Detection for High-Molecular-Weight Polysaccharides in Capillary Electrophoresis</title><author>Richmond, M.D. ; Yeung, E.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c434t-b4855f8b27acc2842926bd48ce30243ccc0abb2f122e43f213fa2eb740bfa5783</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amylose - analogs & derivatives</topic><topic>Amylose - analysis</topic><topic>Amylose - isolation & purification</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Carbohydrates</topic><topic>Dextrans - analysis</topic><topic>Dextrans - isolation & purification</topic><topic>Electrophoresis - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Holosides</topic><topic>Hydrogen-Ion Concentration</topic><topic>Lasers</topic><topic>Microchemistry - methods</topic><topic>Microscopy, Fluorescence - methods</topic><topic>Molecular Weight</topic><topic>Other biological molecules</topic><topic>Polysaccharides - analysis</topic><topic>Polysaccharides - isolation & purification</topic><topic>Sensitivity and Specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Richmond, M.D.</creatorcontrib><creatorcontrib>Yeung, E.S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Richmond, M.D.</au><au>Yeung, E.S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of Laser-Excited Indirect Fluorescence Detection for High-Molecular-Weight Polysaccharides in Capillary Electrophoresis</atitle><jtitle>Analytical biochemistry</jtitle><addtitle>Anal Biochem</addtitle><date>1993-05-01</date><risdate>1993</risdate><volume>210</volume><issue>2</issue><spage>245</spage><epage>248</epage><pages>245-248</pages><issn>0003-2697</issn><eissn>1096-0309</eissn><coden>ANBCA2</coden><abstract>The separation and detection of native polysaccharides in capillary zone electrophoresis is presented. To provide differences in the electrophoretic mobility, a pH of 11.5 is used to partially ionize the analytes. Detection is made possible by indirect fluorescence, where the charged analytes displace the anionic fluorescing buffer ion to create negative peaks. Detection limits in the picogram range are possible.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>7685564</pmid><doi>10.1006/abio.1993.1190</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-2697 |
| ispartof | Analytical biochemistry, 1993-05, Vol.210 (2), p.245-248 |
| issn | 0003-2697 1096-0309 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75777052 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Amylose - analogs & derivatives Amylose - analysis Amylose - isolation & purification Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrates Dextrans - analysis Dextrans - isolation & purification Electrophoresis - methods Fundamental and applied biological sciences. Psychology Holosides Hydrogen-Ion Concentration Lasers Microchemistry - methods Microscopy, Fluorescence - methods Molecular Weight Other biological molecules Polysaccharides - analysis Polysaccharides - isolation & purification Sensitivity and Specificity |
| title | Development of Laser-Excited Indirect Fluorescence Detection for High-Molecular-Weight Polysaccharides in Capillary Electrophoresis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T15%3A58%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20Laser-Excited%20Indirect%20Fluorescence%20Detection%20for%20High-Molecular-Weight%20Polysaccharides%20in%20Capillary%20Electrophoresis&rft.jtitle=Analytical%20biochemistry&rft.au=Richmond,%20M.D.&rft.date=1993-05-01&rft.volume=210&rft.issue=2&rft.spage=245&rft.epage=248&rft.pages=245-248&rft.issn=0003-2697&rft.eissn=1096-0309&rft.coden=ANBCA2&rft_id=info:doi/10.1006/abio.1993.1190&rft_dat=%3Cproquest_cross%3E75777052%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=75777052&rft_id=info:pmid/7685564&rft_els_id=S0003269783711905&rfr_iscdi=true |