Development of Laser-Excited Indirect Fluorescence Detection for High-Molecular-Weight Polysaccharides in Capillary Electrophoresis

The separation and detection of native polysaccharides in capillary zone electrophoresis is presented. To provide differences in the electrophoretic mobility, a pH of 11.5 is used to partially ionize the analytes. Detection is made possible by indirect fluorescence, where the charged analytes displa...

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Veröffentlicht in:	Analytical biochemistry 1993-05, Vol.210 (2), p.245-248
Hauptverfasser:	Richmond, M.D., Yeung, E.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amylose - analogs & derivatives Amylose - analysis Amylose - isolation & purification Analytical, structural and metabolic biochemistry Biological and medical sciences Carbohydrates Dextrans - analysis Dextrans - isolation & purification Electrophoresis - methods Fundamental and applied biological sciences. Psychology Holosides Hydrogen-Ion Concentration Lasers Microchemistry - methods Microscopy, Fluorescence - methods Molecular Weight Other biological molecules Polysaccharides - analysis Polysaccharides - isolation & purification Sensitivity and Specificity
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Beschreibung
Zusammenfassung:	The separation and detection of native polysaccharides in capillary zone electrophoresis is presented. To provide differences in the electrophoretic mobility, a pH of 11.5 is used to partially ionize the analytes. Detection is made possible by indirect fluorescence, where the charged analytes displace the anionic fluorescing buffer ion to create negative peaks. Detection limits in the picogram range are possible.
ISSN:	0003-2697 1096-0309
DOI:	10.1006/abio.1993.1190