The neuroanatomic binding pattern of [ 125I]atrial natriuretic factor in the Japanese quail brain

Application of quantitative autoradiography technique provided a discrete anatomical distribution pattern of the atrial natriuretic factor (ANF) in the Japanese quail, Coturnix coturnix japonica, brain. The highest binding levels of [ 125I]ANF were shown to occur in telencephalon areas, such as fasciculus diagonalis Brocae (232 fmol/mg protein), septum (194 fmol/mg protein) and olfactory bulb (153 fmol/mg protein), and in posterior sites, such as nucleus interpeduncularis (177 fmol/mg protein), while lower levels (> 51 < 87 fmol/mg protein) were found in the hypothalamic sites of the diencephalon. The similar ANF receptor density levels in some brain areas of the quail as well as both mammalian and non-mammalian species suggest that this peptide might be involved in osmoregulatory activities (at the brain level) and furthermore indicate a probable functional conservation of ANF in vertebrates.