Application of Amido black staining to enumerating bacteria grown on membrane filters

A method to detect and enumerate bacterial colonies grown on membrane filters (MF) was described. The colonies were stained with an ethanolic solution of 0.1% Amido black 10B. The procedure yielded the rapid detection of colonies as compared to a conventional plate counting method.

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Bibliographische Detailangaben
Veröffentlicht in:	Journal of microbiological methods 2003-11, Vol.55 (2), p.393-397
1. Verfasser:	Inatomi, Ken-ichi
Format:	Artikel
Sprache:	eng
Schlagworte:	Amido black Amido Black - metabolism Bacillus subtilis - growth & development Bacillus subtilis - isolation & purification Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Colony detection Coloring Agents - metabolism Escherichia coli - growth & development Escherichia coli - isolation & purification Filtration Fundamental and applied biological sciences. Psychology Membrane filter Membranes, Artificial Microbiology Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - isolation & purification Staining Staining and Labeling Water Microbiology
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Beschreibung
Zusammenfassung:	A method to detect and enumerate bacterial colonies grown on membrane filters (MF) was described. The colonies were stained with an ethanolic solution of 0.1% Amido black 10B. The procedure yielded the rapid detection of colonies as compared to a conventional plate counting method.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/S0167-7012(03)00170-2