Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis
Four male cone-specific promoters were isolated from the genome of Pinus radiata D. Don, fused to the beta-glucuronidase (GUS) reporter gene and analysed in the heterologous host Arabidopsis thaliana (L.) Heynh. The temporal and spatial activities of the promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1 d...
Gespeichert in:
| Veröffentlicht in: | Planta 2003-10, Vol.217 (6), p.858-867 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 867 |
|---|---|
| container_issue | 6 |
| container_start_page | 858 |
| container_title | Planta |
| container_volume | 217 |
| creator | Hofig, K.P Moyle, R.L Putterill, J Walter, C |
| description | Four male cone-specific promoters were isolated from the genome of Pinus radiata D. Don, fused to the beta-glucuronidase (GUS) reporter gene and analysed in the heterologous host Arabidopsis thaliana (L.) Heynh. The temporal and spatial activities of the promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1 during seven anther developmental stages are described in detail. The two promoters PrMC2 and PrMALE1 confer an identical GUS expression pattern on Arabidopsis anthers. DNA sequence analysis of the PrMC2 and PrMALE1 promoters revealed an 88% sequence identity over 276 bp and divergence further upstream ( |
| doi_str_mv | 10.1007/s00425-003-1057-9 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_proqu</sourceid><recordid>TN_cdi_proquest_miscellaneous_75733864</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>23388081</jstor_id><sourcerecordid>23388081</sourcerecordid><originalsourceid>FETCH-LOGICAL-c466t-c22b95cb074e6a96d5f0507b99b9cc4a73003c29174f127ec2359d1d5a9d6c243</originalsourceid><addsrcrecordid>eNpdkF9rFDEUxYModq1-AB_UINS30Zt_k-SxlFqFgoL2OWQymW6WmcmaOwvttzfLLBZ8CuH87uGcQ8hbBp8ZgP6CAJKrBkA0DJRu7DOyYVLwhoM0z8mmCrwBK9QZeYW4A6ii1i_JGeNGGMnshnTXD_sSEVOeqZ_9-IgJaR7okA-F_kzzAWnxffKLp5MfIw15jnRf8pSXWJCmmS7bSLex_vKY73PltxkXell8l_q8r3avyYvBjxjfnN5zcvf1-vfVt-b2x833q8vbJsi2XZrAeWdV6EDL2Hrb9moABbqztrMhSK9FrRO4ZVoOjOsYuFC2Z73ytm8Dl-KcfFp9a7w_h4iLmxKGOI5-jjWX00oLYdoj-PE_cFfb1vLoDAejtVWsQmyFQsmIJQ5uX9Lky6Nj4I7ru3V9V1O54_rO1pv3J-NDN8X-6eI0dwUuToDH4Meh-DkkfOIUB6uNqdy7ldvhkss_ndf8Bswx3IdVH3x2_r5Uj7tfHFidyAormBZ_Aew7n4g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>820877951</pqid></control><display><type>article</type><title>Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis</title><source>Jstor Complete Legacy</source><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>Hofig, K.P ; Moyle, R.L ; Putterill, J ; Walter, C</creator><creatorcontrib>Hofig, K.P ; Moyle, R.L ; Putterill, J ; Walter, C</creatorcontrib><description>Four male cone-specific promoters were isolated from the genome of Pinus radiata D. Don, fused to the beta-glucuronidase (GUS) reporter gene and analysed in the heterologous host Arabidopsis thaliana (L.) Heynh. The temporal and spatial activities of the promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1 during seven anther developmental stages are described in detail. The two promoters PrMC2 and PrMALE1 confer an identical GUS expression pattern on Arabidopsis anthers. DNA sequence analysis of the PrMC2 and PrMALE1 promoters revealed an 88% sequence identity over 276 bp and divergence further upstream (<40% sequence identity). GUS expression driven by a 276-bp PrMALE1 promoter fragment showed the same pattern in Arabidopsis anthers as observed for the full-length PrMALE1 promoter. Within the 276-bp promoter fragment a region of high homology to a previously described 16-bp anther-box was identified. In gain-of-function experiments the putative PrMALE1 anther-box was fused upstream of a 90-bp CaMV 35S minimal promoter, as a single copy in the sense direction and as an inverted repeat. No GUS expression was conferred to Arabidopsis anthers by either of these two constructs. In a loss-of-function experiment a 226-bp PrMALE1 deletion construct, which did not contain the putative PrMALE1 anther-box, still maintained the originally observed PrMALE1 GUS expression pattern. Hence, gain-of-function as well as loss-of-function experiments consistently showed that the putative anther-box of the PrMALE1 promoter is non-functional in the Arabidopsis genetic background. For the analysis of the four full-length pine promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1, transformation vectors based on pCAMBIA2200 and pCAMBIA1302 were used. It will also be demonstrated in this article that sequences within the T-DNA borders of these vectors caused a characteristic histological background expression in Arabidopsis, with staining observed in vascular tissue of leaves, sepals, roots, filaments of stamens and in stems and pistils.</description><identifier>ISSN: 0032-0935</identifier><identifier>EISSN: 1432-2048</identifier><identifier>DOI: 10.1007/s00425-003-1057-9</identifier><identifier>PMID: 12838419</identifier><identifier>CODEN: PLANAB</identifier><language>eng</language><publisher>Berlin: Springer-Verlag</publisher><subject>Agronomy. Soil science and plant productions ; Anthers ; Arabidopsis - genetics ; Arabidopsis thaliana ; Base Sequence ; beta-glucuronidase ; Biological and medical sciences ; Cloning, Molecular ; Complementary DNA ; Deoxyribonucleic acid ; Developmental stages ; DNA ; DNA Primers ; flowering ; Fundamental and applied biological sciences. Psychology ; gene deletion ; gene expression ; Genetic vectors ; Genetics and breeding of economic plants ; Glucuronidase - genetics ; Glucuronidase - metabolism ; Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia ; histochemistry ; male flowers ; Male infertility ; Microspores ; Microsporocytes ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequences ; Pinus - genetics ; Pinus - physiology ; Pinus radiata ; plant anatomy ; Plant breeding: fundamental aspects and methodology ; plant proteins ; Plant tissues ; Plants ; Plants, Genetically Modified ; Pollen ; promoter regions ; Promoter Regions, Genetic ; recombinant fusion proteins ; Recombinant Proteins - metabolism ; reporter genes ; Seeds - physiology ; Transcription. Transcription factor. Splicing. Rna processing ; Transgenic plants</subject><ispartof>Planta, 2003-10, Vol.217 (6), p.858-867</ispartof><rights>Springer-Verlag Berlin Heidelberg 2003</rights><rights>2004 INIST-CNRS</rights><rights>Springer-Verlag 2003</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-c22b95cb074e6a96d5f0507b99b9cc4a73003c29174f127ec2359d1d5a9d6c243</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/23388081$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/23388081$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,776,780,799,27903,27904,57995,58228</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15209788$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12838419$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hofig, K.P</creatorcontrib><creatorcontrib>Moyle, R.L</creatorcontrib><creatorcontrib>Putterill, J</creatorcontrib><creatorcontrib>Walter, C</creatorcontrib><title>Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis</title><title>Planta</title><addtitle>Planta</addtitle><description>Four male cone-specific promoters were isolated from the genome of Pinus radiata D. Don, fused to the beta-glucuronidase (GUS) reporter gene and analysed in the heterologous host Arabidopsis thaliana (L.) Heynh. The temporal and spatial activities of the promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1 during seven anther developmental stages are described in detail. The two promoters PrMC2 and PrMALE1 confer an identical GUS expression pattern on Arabidopsis anthers. DNA sequence analysis of the PrMC2 and PrMALE1 promoters revealed an 88% sequence identity over 276 bp and divergence further upstream (<40% sequence identity). GUS expression driven by a 276-bp PrMALE1 promoter fragment showed the same pattern in Arabidopsis anthers as observed for the full-length PrMALE1 promoter. Within the 276-bp promoter fragment a region of high homology to a previously described 16-bp anther-box was identified. In gain-of-function experiments the putative PrMALE1 anther-box was fused upstream of a 90-bp CaMV 35S minimal promoter, as a single copy in the sense direction and as an inverted repeat. No GUS expression was conferred to Arabidopsis anthers by either of these two constructs. In a loss-of-function experiment a 226-bp PrMALE1 deletion construct, which did not contain the putative PrMALE1 anther-box, still maintained the originally observed PrMALE1 GUS expression pattern. Hence, gain-of-function as well as loss-of-function experiments consistently showed that the putative anther-box of the PrMALE1 promoter is non-functional in the Arabidopsis genetic background. For the analysis of the four full-length pine promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1, transformation vectors based on pCAMBIA2200 and pCAMBIA1302 were used. It will also be demonstrated in this article that sequences within the T-DNA borders of these vectors caused a characteristic histological background expression in Arabidopsis, with staining observed in vascular tissue of leaves, sepals, roots, filaments of stamens and in stems and pistils.</description><subject>Agronomy. Soil science and plant productions</subject><subject>Anthers</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis thaliana</subject><subject>Base Sequence</subject><subject>beta-glucuronidase</subject><subject>Biological and medical sciences</subject><subject>Cloning, Molecular</subject><subject>Complementary DNA</subject><subject>Deoxyribonucleic acid</subject><subject>Developmental stages</subject><subject>DNA</subject><subject>DNA Primers</subject><subject>flowering</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene deletion</subject><subject>gene expression</subject><subject>Genetic vectors</subject><subject>Genetics and breeding of economic plants</subject><subject>Glucuronidase - genetics</subject><subject>Glucuronidase - metabolism</subject><subject>Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia</subject><subject>histochemistry</subject><subject>male flowers</subject><subject>Male infertility</subject><subject>Microspores</subject><subject>Microsporocytes</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>Pinus - genetics</subject><subject>Pinus - physiology</subject><subject>Pinus radiata</subject><subject>plant anatomy</subject><subject>Plant breeding: fundamental aspects and methodology</subject><subject>plant proteins</subject><subject>Plant tissues</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Pollen</subject><subject>promoter regions</subject><subject>Promoter Regions, Genetic</subject><subject>recombinant fusion proteins</subject><subject>Recombinant Proteins - metabolism</subject><subject>reporter genes</subject><subject>Seeds - physiology</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Transgenic plants</subject><issn>0032-0935</issn><issn>1432-2048</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2003</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNpdkF9rFDEUxYModq1-AB_UINS30Zt_k-SxlFqFgoL2OWQymW6WmcmaOwvttzfLLBZ8CuH87uGcQ8hbBp8ZgP6CAJKrBkA0DJRu7DOyYVLwhoM0z8mmCrwBK9QZeYW4A6ii1i_JGeNGGMnshnTXD_sSEVOeqZ_9-IgJaR7okA-F_kzzAWnxffKLp5MfIw15jnRf8pSXWJCmmS7bSLex_vKY73PltxkXell8l_q8r3avyYvBjxjfnN5zcvf1-vfVt-b2x833q8vbJsi2XZrAeWdV6EDL2Hrb9moABbqztrMhSK9FrRO4ZVoOjOsYuFC2Z73ytm8Dl-KcfFp9a7w_h4iLmxKGOI5-jjWX00oLYdoj-PE_cFfb1vLoDAejtVWsQmyFQsmIJQ5uX9Lky6Nj4I7ru3V9V1O54_rO1pv3J-NDN8X-6eI0dwUuToDH4Meh-DkkfOIUB6uNqdy7ldvhkss_ndf8Bswx3IdVH3x2_r5Uj7tfHFidyAormBZ_Aew7n4g</recordid><startdate>20031001</startdate><enddate>20031001</enddate><creator>Hofig, K.P</creator><creator>Moyle, R.L</creator><creator>Putterill, J</creator><creator>Walter, C</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QP</scope><scope>7QR</scope><scope>7TM</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20031001</creationdate><title>Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis</title><author>Hofig, K.P ; Moyle, R.L ; Putterill, J ; Walter, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-c22b95cb074e6a96d5f0507b99b9cc4a73003c29174f127ec2359d1d5a9d6c243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2003</creationdate><topic>Agronomy. Soil science and plant productions</topic><topic>Anthers</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis thaliana</topic><topic>Base Sequence</topic><topic>beta-glucuronidase</topic><topic>Biological and medical sciences</topic><topic>Cloning, Molecular</topic><topic>Complementary DNA</topic><topic>Deoxyribonucleic acid</topic><topic>Developmental stages</topic><topic>DNA</topic><topic>DNA Primers</topic><topic>flowering</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene deletion</topic><topic>gene expression</topic><topic>Genetic vectors</topic><topic>Genetics and breeding of economic plants</topic><topic>Glucuronidase - genetics</topic><topic>Glucuronidase - metabolism</topic><topic>Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia</topic><topic>histochemistry</topic><topic>male flowers</topic><topic>Male infertility</topic><topic>Microspores</topic><topic>Microsporocytes</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequences</topic><topic>Pinus - genetics</topic><topic>Pinus - physiology</topic><topic>Pinus radiata</topic><topic>plant anatomy</topic><topic>Plant breeding: fundamental aspects and methodology</topic><topic>plant proteins</topic><topic>Plant tissues</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Pollen</topic><topic>promoter regions</topic><topic>Promoter Regions, Genetic</topic><topic>recombinant fusion proteins</topic><topic>Recombinant Proteins - metabolism</topic><topic>reporter genes</topic><topic>Seeds - physiology</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Transgenic plants</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hofig, K.P</creatorcontrib><creatorcontrib>Moyle, R.L</creatorcontrib><creatorcontrib>Putterill, J</creatorcontrib><creatorcontrib>Walter, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Planta</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hofig, K.P</au><au>Moyle, R.L</au><au>Putterill, J</au><au>Walter, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis</atitle><jtitle>Planta</jtitle><addtitle>Planta</addtitle><date>2003-10-01</date><risdate>2003</risdate><volume>217</volume><issue>6</issue><spage>858</spage><epage>867</epage><pages>858-867</pages><issn>0032-0935</issn><eissn>1432-2048</eissn><coden>PLANAB</coden><abstract>Four male cone-specific promoters were isolated from the genome of Pinus radiata D. Don, fused to the beta-glucuronidase (GUS) reporter gene and analysed in the heterologous host Arabidopsis thaliana (L.) Heynh. The temporal and spatial activities of the promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1 during seven anther developmental stages are described in detail. The two promoters PrMC2 and PrMALE1 confer an identical GUS expression pattern on Arabidopsis anthers. DNA sequence analysis of the PrMC2 and PrMALE1 promoters revealed an 88% sequence identity over 276 bp and divergence further upstream (<40% sequence identity). GUS expression driven by a 276-bp PrMALE1 promoter fragment showed the same pattern in Arabidopsis anthers as observed for the full-length PrMALE1 promoter. Within the 276-bp promoter fragment a region of high homology to a previously described 16-bp anther-box was identified. In gain-of-function experiments the putative PrMALE1 anther-box was fused upstream of a 90-bp CaMV 35S minimal promoter, as a single copy in the sense direction and as an inverted repeat. No GUS expression was conferred to Arabidopsis anthers by either of these two constructs. In a loss-of-function experiment a 226-bp PrMALE1 deletion construct, which did not contain the putative PrMALE1 anther-box, still maintained the originally observed PrMALE1 GUS expression pattern. Hence, gain-of-function as well as loss-of-function experiments consistently showed that the putative anther-box of the PrMALE1 promoter is non-functional in the Arabidopsis genetic background. For the analysis of the four full-length pine promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1, transformation vectors based on pCAMBIA2200 and pCAMBIA1302 were used. It will also be demonstrated in this article that sequences within the T-DNA borders of these vectors caused a characteristic histological background expression in Arabidopsis, with staining observed in vascular tissue of leaves, sepals, roots, filaments of stamens and in stems and pistils.</abstract><cop>Berlin</cop><pub>Springer-Verlag</pub><pmid>12838419</pmid><doi>10.1007/s00425-003-1057-9</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0032-0935 |
| ispartof | Planta, 2003-10, Vol.217 (6), p.858-867 |
| issn | 0032-0935 1432-2048 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75733864 |
| source | Jstor Complete Legacy; MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | Agronomy. Soil science and plant productions Anthers Arabidopsis - genetics Arabidopsis thaliana Base Sequence beta-glucuronidase Biological and medical sciences Cloning, Molecular Complementary DNA Deoxyribonucleic acid Developmental stages DNA DNA Primers flowering Fundamental and applied biological sciences. Psychology gene deletion gene expression Genetic vectors Genetics and breeding of economic plants Glucuronidase - genetics Glucuronidase - metabolism Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia histochemistry male flowers Male infertility Microspores Microsporocytes Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequences Pinus - genetics Pinus - physiology Pinus radiata plant anatomy Plant breeding: fundamental aspects and methodology plant proteins Plant tissues Plants Plants, Genetically Modified Pollen promoter regions Promoter Regions, Genetic recombinant fusion proteins Recombinant Proteins - metabolism reporter genes Seeds - physiology Transcription. Transcription factor. Splicing. Rna processing Transgenic plants |
| title | Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-28T00%3A22%3A34IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_proqu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20analysis%20of%20four%20Pinus%20radiata%20male%20cone%20promoters%20in%20the%20heterologous%20host%20Arabidopsis&rft.jtitle=Planta&rft.au=Hofig,%20K.P&rft.date=2003-10-01&rft.volume=217&rft.issue=6&rft.spage=858&rft.epage=867&rft.pages=858-867&rft.issn=0032-0935&rft.eissn=1432-2048&rft.coden=PLANAB&rft_id=info:doi/10.1007/s00425-003-1057-9&rft_dat=%3Cjstor_proqu%3E23388081%3C/jstor_proqu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=820877951&rft_id=info:pmid/12838419&rft_jstor_id=23388081&rfr_iscdi=true |