Expression analysis of four Pinus radiata male cone promoters in the heterologous host Arabidopsis

Four male cone-specific promoters were isolated from the genome of Pinus radiata D. Don, fused to the beta-glucuronidase (GUS) reporter gene and analysed in the heterologous host Arabidopsis thaliana (L.) Heynh. The temporal and spatial activities of the promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1 d...

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Veröffentlicht in:	Planta 2003-10, Vol.217 (6), p.858-867
Hauptverfasser:	Hofig, K.P, Moyle, R.L, Putterill, J, Walter, C
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Anthers Arabidopsis - genetics Arabidopsis thaliana Base Sequence beta-glucuronidase Biological and medical sciences Cloning, Molecular Complementary DNA Deoxyribonucleic acid Developmental stages DNA DNA Primers flowering Fundamental and applied biological sciences. Psychology gene deletion gene expression Genetic vectors Genetics and breeding of economic plants Glucuronidase - genetics Glucuronidase - metabolism Heterosis. Floral biology applications: apomixy, male sterility, incompatibility, varia histochemistry male flowers Male infertility Microspores Microsporocytes Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequences Pinus - genetics Pinus - physiology Pinus radiata plant anatomy Plant breeding: fundamental aspects and methodology plant proteins Plant tissues Plants Plants, Genetically Modified Pollen promoter regions Promoter Regions, Genetic recombinant fusion proteins Recombinant Proteins - metabolism reporter genes Seeds - physiology Transcription. Transcription factor. Splicing. Rna processing Transgenic plants
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Zusammenfassung:	Four male cone-specific promoters were isolated from the genome of Pinus radiata D. Don, fused to the beta-glucuronidase (GUS) reporter gene and analysed in the heterologous host Arabidopsis thaliana (L.) Heynh. The temporal and spatial activities of the promoters PrCHS1, PrLTP2, PrMC2 and PrMALE1 during seven anther developmental stages are described in detail. The two promoters PrMC2 and PrMALE1 confer an identical GUS expression pattern on Arabidopsis anthers. DNA sequence analysis of the PrMC2 and PrMALE1 promoters revealed an 88% sequence identity over 276 bp and divergence further upstream (
ISSN:	0032-0935 1432-2048
DOI:	10.1007/s00425-003-1057-9