Osteoblastic differentiation of cultured rat bone marrow cells on hydroxyapatite with different surface topography

Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. Objective. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on the response of the rat bone marrow cells, evaluating: cell att...

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Veröffentlicht in:	Dental materials 2003-12, Vol.19 (8), p.768-772
Hauptverfasser:	Rosa, Adalberto L, Beloti, Márcio M, van Noort, Richard
Format:	Artikel
Sprache:	eng
Schlagworte:	Alkaline Phosphatase - analysis Analysis of Variance Animals Biocompatible Materials - chemistry Bone Marrow Cells - physiology Cell Adhesion Cell culture Cell Differentiation Cell Division Cells, Cultured Dentistry Durapatite - chemistry Hydroxyapatite Male Microporosity Osteoblasts - physiology Osteogenesis - physiology Porosity Proteins - analysis Rat bone marrow cells Rats Rats, Wistar Surface Properties Surface topography
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Beschreibung
Zusammenfassung:	Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. Objective. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on the response of the rat bone marrow cells, evaluating: cell attachment, proliferation, total protein content, alkaline phosphatase (ALP) activity, and bone-like nodule formation. Methods. Cells were cultured on HA discs manufactured by a combination of uniaxial powder pressing and different sintering conditions, with different percentage of microporosity (
ISSN:	0109-5641 1879-0097
DOI:	10.1016/S0109-5641(03)00024-1