Regulation of the expression of the Pseudomonas stutzeri recA gene

With the aid of recA-lacZ fusion strains, the in vivo regulation of the Pseudomonas stutzeri recA gene has been studied. It is shown that expression of this gene can be induced with a variety of DNA damaging agents, as well as with agents that interfere with DNA replication. For this induction, the...

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Veröffentlicht in:	Antonie van Leeuwenhoek 1993-01, Vol.63 (1), p.55-62
Hauptverfasser:	Vosman, B, Rauch, P J, Westerhoff, H V, Hellingwerf, K J
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Base Sequence beta-Galactosidase - biosynthesis Cloning, Molecular DNA Damage DNA, Bacterial - drug effects DNA, Bacterial - genetics Gene Expression Regulation, Bacterial - drug effects Genes, Bacterial Lac Operon Molecular Sequence Data Plasmids Pseudomonas - enzymology Pseudomonas - genetics Pseudomonas stutzeri Rec A Recombinases - genetics
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Zusammenfassung:	With the aid of recA-lacZ fusion strains, the in vivo regulation of the Pseudomonas stutzeri recA gene has been studied. It is shown that expression of this gene can be induced with a variety of DNA damaging agents, as well as with agents that interfere with DNA replication. For this induction, the presence of an active RecA protein is essential. Sequence analysis of the promoter region of the P. stutzeri recA gene showed that its open reading frame is preceded by an SOS-box, suggesting a regulation of its expression, similar to the regulation of recA expression in Escherichia coli.
ISSN:	0003-6072
DOI:	10.1007/BF00871732