Conversion of starch to ethanol in a recombinant Saccharomyces cerevisiae strain expressing rice alpha-amylase from a novel Pichia pastoris alcohol oxidase promoter

A recombinant Saccharomyces cerevisiae , expressing and secreting rice α -amylase, converts starch to ethanol. The rice α -amylase gene ( OS103 ) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequence...

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Veröffentlicht in:	Bio/Technology 1993, Vol.11 (5), p.606-610
Hauptverfasser:	Kumagai, Monto H., Sverlow, Genadie G., della-Cioppa, Guy, Grill, Laurence K.
Format:	Artikel
Sprache:	eng
Schlagworte:	actividad enzimatica activite enzymatique adn recombinado adn recombine Agriculture alcohol dehydrogenase alcohol deshidrogenasa Alcohol Oxidoreductases - genetics alcool deshydrogenase alfa amilasa almidon alpha amylase alpha-Amylases - genetics alpha-Amylases - metabolism amidon Amino Acid Sequence Base Sequence Bioinformatics Biological and medical sciences Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology carbohydrate metabolism Cloning, Molecular Conserved Sequence DNA, Fungal - chemistry DNA, Fungal - genetics enzymic activity Escherichia coli - genetics etanol ethanol Ethanol - metabolism expresion genica expression des genes Fundamental and applied biological sciences. Psychology gene Gene Expression genes Genes, Fungal Genetic engineering Genetic technics Life Sciences metabolisme des glucides metabolismo de carbohidratos Methods. Procedures. Technologies Molecular Sequence Data nucleotide sequence Oryza - enzymology Oryza - genetics pichia Pichia - enzymology Pichia - genetics produccion production Promoter Regions, Genetic proteinas proteine proteins recombinant dna saccharomyces cerevisiae Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae - metabolism secrecion secretion secuencia nucleica sequence nucleique starch Starch - metabolism Transformation, Bacterial Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Zusammenfassung:	A recombinant Saccharomyces cerevisiae , expressing and secreting rice α -amylase, converts starch to ethanol. The rice α -amylase gene ( OS103 ) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequences of ZZA1 and other methanol-regulated promoters were analyzed. A highly conserved sequence (TTG-N 3 GCTTCCAA-N 5 -TGGT) was found in the 5′ flanking regions of alcohol oxidase, methanol oxidase, and dihydroxyacetone synthase genes in Pichia pastoris, Hansenula polymorpha , and Candida boidinii S2. The yeast strain containing the ZZA1-OS103 fusion secreted biologically active enzyme into the culture media while fermenting soluble starch.
ISSN:	0733-222X 1087-0156 2331-3684 1546-1696
DOI:	10.1038/nbt0593-606