Mass spectrometry in the diagnosis of steroid-related disorders and in hypertension research

The use of GC/MS and microbore HPLC/electrospray mass spectrometry for clinical studies in hypertension and mineralocorticoid research is described. In particular, an automated GC/MS method allows nearly quantitative measurements of metabolites of major steroids of adrenal, placental and gonadal ori...

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Veröffentlicht in:	Journal of steroid biochemistry and molecular biology 1993-04, Vol.45 (1-3), p.127-140
1. Verfasser:	Shackleton, Cedric H.L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adrenal Gland Neoplasms - metabolism Adrenal Hyperplasia, Congenital Adult Biological and medical sciences Carbohydrate Sequence Child Child, Preschool Chromatography, High Pressure Liquid Endocrinology Female Gas Chromatography-Mass Spectrometry Glucocorticoids - metabolism Glycyrrhiza - metabolism Humans Hyperaldosteronism - metabolism Hypertension - diagnosis Infant Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Middle Aged Mineralocorticoids - metabolism Molecular Sequence Data Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Plants, Medicinal Pseudohypoaldosteronism - metabolism Reference Values Steroids - metabolism
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Zusammenfassung:	The use of GC/MS and microbore HPLC/electrospray mass spectrometry for clinical studies in hypertension and mineralocorticoid research is described. In particular, an automated GC/MS method allows nearly quantitative measurements of metabolites of major steroids of adrenal, placental and gonadal origin. This method is able to distinguish almost all steroid related disorders. Electrospray mass spectrometry in conjunction with microbore HPLC is the latest mass spectrometry technique applicable to biochemical investigations. It offers high sensitivity (15 pg) for measurement of intact steroid conjugates and related compounds.
ISSN:	0960-0760 1879-1220
DOI:	10.1016/0960-0760(93)90132-G